C1orf41 Methods: Difference between revisions
From MDWiki
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI) | The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI) webpage and was used to find homologues of this protein using Blastp non-redundant protein database. Amino acid Sequence of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed poor alignment were discarded from further investigation. From the sequence alignment, a phylogenetic tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates. | ||
[[Chromosome 1 open reading frame 41|Back to main page]] | [[Chromosome 1 open reading frame 41|Back to main page]] |
Revision as of 11:22, 9 June 2009
The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI) webpage and was used to find homologues of this protein using Blastp non-redundant protein database. Amino acid Sequence of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed poor alignment were discarded from further investigation. From the sequence alignment, a phylogenetic tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates.