Results - 2gqnA: Difference between revisions
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There are two main domains regarding 2qgnA. | There are two main domains regarding 2qgnA. | ||
2qgnA is composed of two main domains. CATH analysis of 2qgn resulted in the finding of two main domains composing 2qgnA. | |||
Domain 1 ranges from residue 2-200 and residue 283-314. Domain 2 encompasses residues from 201-282. | |||
[[Image:domain pic3.jpg|framed|'''Figure 4''' <BR>Two main domains exhibited by tRNA isopentenyl transferase(2qgnA). Blue regions denote first domain while Red regions underlies second domain.|none]]<BR> | |||
[[Image:domain--1.jpg|framed|'''Figure 5'''<BR> Structural architecture of domain 2 signified by red regions in Figure 4.|left]]<BR> | |||
[[Image:domain--2.jpg|framed|'''Figure 6'''<BR> Structural representation of domain 1 denoted by blue regions in Figure 4.|none]]<BR> | |||
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-Functional Sites Found by Sequence Conservation In Structurally Related Proteins | -Functional Sites Found by Sequence Conservation In Structurally Related Proteins | ||
-Functional Sites Found by Structure Conservation In Structurally Related Proteins | -Functional Sites Found by Structure Conservation In Structurally Related Proteins | ||
==Multiple Sequence Alignment== | ==Multiple Sequence Alignment== | ||
==Tree== | ==Tree== |
Revision as of 05:03, 3 June 2008
Structure of tRNA isopentenyltransferase
Protein Sequence in FASTA format
>gi|152149497|pdb|2QGN|A Chain A, Crystal Structure Of Trna Isopentenylpyrophosphate Transferase (Bh2366) From Bacillus Halodurans, Northeast Structural Genomics Consortium Target Bhr41. XKEKLVAIVGPTAVGKTKTSVXLAKRLNGEVISGDSXQVYRGXDIGTAKITAEEXDGVPHHLIDIKDPSE SFSVADFQDLATPLITEIHERGRLPFLVGGTGLYVNAVIHQFNLGDIRADEDYRHELEAFVNSYGVQALH DKLSKIDPKAAAAIHPNNYRRVIRALEIIKLTGKTVTEQARHEEETPSPYNLVXIGLTXERDVLYDRINR RVDQXVEEGLIDEAKKLYDRGIRDCQSVQAIGYKEXYDYLDGNVTLEEAIDTLKRNSRRYAKRQLTWFRN KANVTWFDXTDVDFDKKIXEIHNFIAGKLEEKSKLEHHHHHH
Protein Structure
Secondary Structure
Analysis of the secondary structure acquired from Protein Data Bank showed results as displayed below :
Surface Properties of 2qgn
Ligand Binding Sites and Surface Clefts
Localisation Expression of tRNA isopentenyltransferase
Generally, this enzyme is expressed in all tissue types since it is important that functional protein are synthesized in each of these tissues. Specifically, it is highly expressed in adipose tissues as well as oocytes. Relatively high amounts of this enzyme is expressed in prostate, adrenal gland, B-cells and trachea. The reason why tRNA-IPT are at higher concentrations in these tissues may reflect higher levels of protein synthesis.
Domain and Structural Analysis
There are two main domains regarding 2qgnA.
2qgnA is composed of two main domains. CATH analysis of 2qgn resulted in the finding of two main domains composing 2qgnA.
Domain 1 ranges from residue 2-200 and residue 283-314. Domain 2 encompasses residues from 201-282.
-Functional Sites Found by Sequence Conservation In Structurally Related Proteins
-Functional Sites Found by Structure Conservation In Structurally Related Proteins