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| == Blast ==
| | [[Image:BLAST.png|centre|framed|'''Figure 1:''' BLAST result]] |
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| [[Image:BLAST.png]]
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| The above shows the result from BLASTP search. The sequences of these proteins are aligned to observe the conserved regions.
| | H-(2x)-W-(29x)-R-(2x)-S-(2x)-E residues critical for galactose binding site |
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| == ClustalX ==
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| Multiple sequence alignment with all of the 27 sequences obtained from BLAST is shown below:
| | [[Image:Msa16.png|centre|framed|'''Figure 2:''' Conserved region of alpha B domain.]] |
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| [[Image:Platypus.png]] | | [[Image:Msa17.png|centre|framed|'''Figure 3:''' Residues involved in binding of Ca2+ and possible binding site.]] |
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| The sequence of platypus, urchin_156 and urchin_159 was removed due to poor alignment. Besides that, the sequence for g.alga_fap was removed as it was a subsequence of g.alga_itp25.
| | [[Image:Treecolour.png|centre|framed|'''Figure 4:''' Phylogenetic tree of c1orf41 with its homologues. Red is marine eukaryote, blue is amphibian and green is terrestrial eukaryotes.]] |
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| | [[Image:Tree.png|centre|framed|'''Figure 5:''' Phylogenetic tree with bootstrap values]] |
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| [[Image:Msafinal.png]]
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| == Phylogenetic Tree ==
| | [[Chromosome 1 open reading frame 41 Function|Next]] |
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| After obtaining the multiple sequence alignment, phylogenetic tree was construct based on the result from multiple sequence alignment. Bootstrap values are shown on each branch.
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| [[Image:Tree.png]] | |
Latest revision as of 06:26, 16 June 2009
Figure 1: BLAST result
H-(2x)-W-(29x)-R-(2x)-S-(2x)-E residues critical for galactose binding site
Figure 2: Conserved region of alpha B domain.
Figure 3: Residues involved in binding of Ca2+ and possible binding site.
Figure 4: Phylogenetic tree of c1orf41 with its homologues. Red is marine eukaryote, blue is amphibian and green is terrestrial eukaryotes.
Figure 5: Phylogenetic tree with bootstrap values
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