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| [[Image:Mouse gene atlas.png]]
| | '''Function from its name''' |
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| | [[Image:reaction1.png|framed|center|none]] |
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| [[Image:Human geneAtlas.png]] | | [[How the enzyme act on tRNA| Next]] |
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| Transferases are enzymes which transfer a group from a donor molecule to an acceptor molecule. As the name suggest, tRNA-IPT adds adenine to tRNA molecules specifically to tRNA, whose anticodon binds to the codon encoded with uridine. Below is the reaction catalysed by tRNA isopentenyltransferase.
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| Isopentenyl diphosphate + tRNA <=> diphosphate + tRNA containing 6-isopentenyladenosine
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| reaction taken from http://www.expasy.org/cgi-bin/nicezyme.pl?2.5.1.8
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| tRNA molecules with modified adenine specifically at position 37 are known as cytokinins.This modified adenine is found next to the anticodon that binds to the codon encoding for uridine.
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| This detailed and precise adenine modification is important in maintaining correct framework and the subsequent translation pathway.
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| Cytokinins are involved in several biochemical and cellular processes such as cell division and growth, both in plants and animals. They can exist in two forms; the tRNA-free and the tRNA-bound form, through addition of adenine to tRNA or AMP acceptor molecules.
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| using the motif search, The C2-H2 Zinc finger motif found in tRNA-IPT also contributes to the enzyme's function. This motif is commonly found in nucleic acid-binding proteins and is composed of 25 to 30 amino acid residues.
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| A schematic representation of a zinc finger domain is shown below:
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| x x
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| x x
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| x x
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| x x
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| x x
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| x x
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| C H
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| x \ / x
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| x Zn x
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| x / \ x
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| C H
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| x x x x x x x x x x
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| http://ca.expasy.org/cgi-bin/nicedoc.pl?PDOC00028
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| The biological process of tRNA-IPT is trna metabolism. Generally, tRNAs are RNA molecules which is involved protein synthesis. They carry amino acids which corresponds to their anti-codon recognition sequence and adds them to the complementary codons along the length of the mRNA.
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