3bsqA Introduction: Difference between revisions
No edit summary |
No edit summary |
||
Line 2: | Line 2: | ||
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as '''EC 3.1.6.''' in enzyme classifications. Sulfatases are found in lysosomes (Peters and ''et al.'', 1990) They all participate in metabolic processes, most of the family members are shown to contain a highly conserved cystine residue and a bivalent metal binding site; but however the molecular and biological function of some of the family members are not yet fully defined. The sulfatase enzyme in our focud is arylsulfatase K, were the crystal structure is been solves, but no functional characterisation is done to the date. In this experiment we looked at proteins with sequence conservation, structural similarity, other proteins that arylsulfatase K is known to interact with, and the level of conservation of some key residues that may be involved in its catalytic actuvity; to determine the possible function of the protein. | |||
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as '''EC 3.1.6.''' in enzyme classifications. They all participate in metabolic processes, most of the family members are shown to contain a highly conserved cystine residue and a bivalent metal binding site; but however the molecular and biological function of some of the family members are not yet fully defined. The sulfatase enzyme in our focud is arylsulfatase K, were the crystal structure is been solves, but no functional characterisation is done to the date. In this experiment we looked at proteins with sequence conservation, structural similarity, other proteins that arylsulfatase K is known to interact with, and the level of conservation of some key residues that may be involved in its catalytic actuvity; to determine the possible function of the protein. | |||
Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB),also a lysosomal enzyme, which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate. | Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB),also a lysosomal enzyme, which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate. |
Revision as of 00:01, 2 June 2008
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as EC 3.1.6. in enzyme classifications. Sulfatases are found in lysosomes (Peters and et al., 1990) They all participate in metabolic processes, most of the family members are shown to contain a highly conserved cystine residue and a bivalent metal binding site; but however the molecular and biological function of some of the family members are not yet fully defined. The sulfatase enzyme in our focud is arylsulfatase K, were the crystal structure is been solves, but no functional characterisation is done to the date. In this experiment we looked at proteins with sequence conservation, structural similarity, other proteins that arylsulfatase K is known to interact with, and the level of conservation of some key residues that may be involved in its catalytic actuvity; to determine the possible function of the protein.
Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB),also a lysosomal enzyme, which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate.