Pyridoxal Phosphatase Methods: Difference between revisions
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'''DALI''' <BR> | '''DALI''' <BR> | ||
A search was carried out using the PDB file (obtained from the previous step) of Pyridoxal Phosphatase. The results were generated and returned via e-mail. Due its similarity to 2cfsA, 2oycA - which was | A search was carried out using the PDB file (obtained from the previous step) of Pyridoxal Phosphatase. The results were generated and returned via e-mail. Due its similarity to 2cfsA, 2oycA - which was at the top of the list of hits generated by the DALI server, was structurally superimposed against 2cfsA using the PyMOL software. | ||
Function | Function | ||
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Revision as of 12:28, 21 May 2008
Evolution
Structure
Protein Data Bank (PDB)
The structure of 2cfsA was obtained from the PDB. A search on the database using Pyridoxal Phosphatase's PDB ID (2cfsA) did not yield any results. A search using the PDB ID "2cfs" was successful. The crystal structure of Pyridoxal Phosphatase was obtained from the "Images and Visualization" section located on the right side of website.
http://www.rcsb.org/pdb/explore/explore.do?structureId=2cfs
The PDB file of Pyridoxal Phosphatase was saved for further use.
DALI
A search was carried out using the PDB file (obtained from the previous step) of Pyridoxal Phosphatase. The results were generated and returned via e-mail. Due its similarity to 2cfsA, 2oycA - which was at the top of the list of hits generated by the DALI server, was structurally superimposed against 2cfsA using the PyMOL software.
Function