Arylformamidase: Difference between revisions
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'''Conservation of Catalytic Triad''' | '''Conservation of Catalytic Triad''' | ||
An alignment of 2pbl and the protein of interest was performed using ClustalX | An alignment of 2pbl and the protein of interest was performed using ClustalX with default parameters. Residues of the catalytic triad as described by the paper characterising it were located in the sequence. Conservation of the residue and sequence surrounding it was observed. | ||
== Additional Materials == | == Additional Materials == |
Revision as of 10:21, 31 May 2008
Abstract
Introduction
Results
Discussion
Conclusion
Methods
Literature search
A literature search was performed using the putative annotation ‘arylformamidase’. A paper by Pabarcus et al. 2007 was returned which, ironically, described the arylformamidase from Mus Musculus.
Conservation of Catalytic Triad
An alignment of 2pbl and the protein of interest was performed using ClustalX with default parameters. Residues of the catalytic triad as described by the paper characterising it were located in the sequence. Conservation of the residue and sequence surrounding it was observed.
Additional Materials
Presentations
Sequence & Homology - Sebastian Mynott
Structure - Basma Al Alaiwat
Function - Thomas Parker
Links
Protein Data Bank Entry for 2PBL
FASTA Sequence
>gi|146387357|pdb|2PBL|A Chain A, Crystal Structure Of Putative Thioesterase (Yp_614486.1) From Silicibacter Sp. Tm1040 At 1.79 A Resolution GXELDDAYANGAYIEGAADYPPRWAASAEDFRNSLQDRARLNLSYGEGDRHKFDLFLPEGTPVGLFVFVH GGYWXAFDKSSWSHLAVGALSKGWAVAXPSYELCPEVRISEITQQISQAVTAAAKEIDGPIVLAGHSAGG HLVARXLDPEVLPEAVGARIRNVVPISPLSDLRPLLRTSXNEKFKXDADAAIAESPVEXQNRYDAKVTVW VGGAERPAFLDQAIWLVEAWDADHVIAFEKHHFNVIEPLADPESDLVAVITA
References
Pabarcus MK, Casida JE. Cloning, expression, and catalytic triad of recombinant arylformamidase. Protein Expr Purif. Nov;44(1):39-44.
De Simone G, Menchise V, Manco G, Mandrich L, Sorrentino N, Lang D, Rossi M, Pedone C. The crystal structure of a hyper-thermophilic carboxylesterase from the archaeon Archaeoglobus fulgidus. J Mol Biol. 2001 Nov 30;314(3):507-18.
Byun JS, Rhee JK, Kim ND, Yoon J, Kim DU, Koh E, Oh JW, Cho HS. Crystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability properties. BMC Struct Biol. 2007 Jul 12;7:47.
2-Effect of arylformamidase (kynurenine formamidase) gene inactivation in mice on enzymatic activity, kynurenine pathway metabolites and phenotype
http://www.ncbi.nlm.nih.gov/pubmed/12007602?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_Discovery_RA&linkpos=2&log$=relatedarticles&dbfrom=pubmed