Results ERp18
Evoltuion of MIF4G Domain Containing Protein
Results notes
First identified in Dario rerio (zebra fish)
Eukaryotic gene - derived gene
Protein conserved throughout the Metazoans (animals)
Structure of MIF4G Domain Containing Protein
From the PDB search, the structure was revealed to be similar to “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein. The protein was isolated form Danio rerio and expressed in Escherichia coli. It is formed from two chains, with two chemical components, nickel (Ni2+) and selenomethionine (C5H11NO2Se) as additions to the protein. The NCBI Entrez search revealed the protein to be a domain of the eIF4G-like protein.
Both Pfam and InterPro identified the protein as the Middle domain of eIF4G, termed MIF4G. MIF4G consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.