C1orf41 Methods
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The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI) webpage and was used to find homologues of this protein using Blastp non-redundant protein database. Amino acid Sequence of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed poor alignment were discarded from further investigation. From the sequence alignment, a phylogenetic tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates.