2gnx BlastP

Mouse Protein Subcellular Localization Database

BLAST Results

BLASTP 2.2.12 [Aug-07-2005]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.

Query=

        (323 letters)

Database: RIKEN IPS7 Protein Sequences

          33,451 sequences; 15,064,402 total letters

Searching..................................................done

E = has experimental SCL data L = has literature-mined SCL data O = has SCL data from external resources P = has predicted SCL data

• • • denotes a protein that maps, with high-confidence, to a transcriptional unit that may exhibit variable membrane organization.

                                                                Score    E

Sequences producing significant alignments: (bits) Value SCL Data Protein Class

PA157757.1|Unknown (protein for MGC:86070)\ 597 e-171 P Soluble, non-secreted protein PA157757.12|hypothetical protein\ 551 e-157 P Soluble, non-secreted protein PA157757.2|hypothetical protein\ 230 1e-60 P Type II membrane protein PA72427.1|neuronal apoptosis inhibitory protein\ 30 3.1 OP Soluble, non-secreted protein PA177473.19|RalGDS-like factor\ 29 5.3 OP Soluble, non-secreted protein PA82764.1|YY1 transcription factor\ 29 5.3 LOP Soluble, non-secreted protein

>PA157757.1|Unknown (protein for MGC:86070)\

         Length = 445

Score =  597 bits (1538), Expect = e-171
Identities = 299/323 (92%), Positives = 299/323 (92%)

Query: 1 PHLSEQLCFFVQARXEIADFYEKXYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP 60

          PHLSEQLCFFVQAR EIADFYEK YALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP

Sbjct: 123 PHLSEQLCFFVQARMEIADFYEKMYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP 182

Query: 61 LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG 120

          LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG

Sbjct: 183 LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG 242

Query: 121 GQSQKAVQPPHXXXXXXXXXXXXXXXXXXYFHEALSRQTTASEXKALTAKANPDLFGKIS 180

          GQSQKAVQPPH                  YFHEALSRQTTASE KALTAKANPDLFGKIS

Sbjct: 243 GQSQKAVQPPHLFLWLMKLKTMLLAKFSFYFHEALSRQTTASEMKALTAKANPDLFGKIS 302

Query: 181 SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVXHWP 240

          SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPV HWP

Sbjct: 303 SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVMHWP 362

Query: 241 NVIXIXTDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFISF 300

          NVI I TDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFISF

Sbjct: 363 NVIMIMTDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFISF 422

Query: 301 LNELSLALKNPKVFASLKPGSKG 323

          LNELSLALKNPKVFASLKPGSKG

Sbjct: 423 LNELSLALKNPKVFASLKPGSKG 445

>PA157757.12|hypothetical protein\

         Length = 430

Score =  551 bits (1419), Expect = e-157
Identities = 275/299 (91%), Positives = 275/299 (91%)

Query: 1 PHLSEQLCFFVQARXEIADFYEKXYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP 60

          PHLSEQLCFFVQAR EIADFYEK YALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP

Sbjct: 123 PHLSEQLCFFVQARMEIADFYEKMYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSP 182

Query: 61 LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG 120

          LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG

Sbjct: 183 LESSFQLEVGVLSHLLKAQAQISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFG 242

Query: 121 GQSQKAVQPPHXXXXXXXXXXXXXXXXXXYFHEALSRQTTASEXKALTAKANPDLFGKIS 180

          GQSQKAVQPPH                  YFHEALSRQTTASE KALTAKANPDLFGKIS

Sbjct: 243 GQSQKAVQPPHLFLWLMKLKTMLLAKFSFYFHEALSRQTTASEMKALTAKANPDLFGKIS 302

Query: 181 SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVXHWP 240

          SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPV HWP

Sbjct: 303 SFIRKYDAANVSLIFDNRGSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVMHWP 362

Query: 241 NVIXIXTDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFIS 299

          NVI I TDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFIS

Sbjct: 363 NVIMIVTDRASDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVVIFESKKSERDSHFIS 421

>PA157757.2|hypothetical protein\

         Length = 261

Score =  230 bits (586), Expect = 1e-60
Identities = 116/135 (85%), Positives = 116/135 (85%)

Query: 21 YEKXYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSPLESSFQLEVGVLSHLLKAQA 80

          YEK YALSTQKFINTEELVSTLDTILRKYSSRFHHPILSPLESSFQLEVGVLSHLLKAQA

Sbjct: 87 YEKMYALSTQKFINTEELVSTLDTILRKYSSRFHHPILSPLESSFQLEVGVLSHLLKAQA 146

Query: 81 QISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFGGQSQKAVQPPHXXXXXXXXX 140

          QISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFGGQSQKAVQPPH         

Sbjct: 147 QISEWKFLPSLVTLHNAHTKLQSWGQTFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLK 206

Query: 141 XXXXXXXXXYFHEAL 155

                   YFHEAL

Sbjct: 207 TMLLAKFSFYFHEAL 221

>PA72427.1|neuronal apoptosis inhibitory protein\

         Length = 1447

Score = 29.6 bits (65), Expect = 3.1
Identities = 31/111 (27%), Positives = 51/111 (45%), Gaps = 17/111 (15%)

Query: 208 YHHPHSY---REAPKGVDQYPAVVS-LPSDRPVXHWPNVIXIXTDRASDLNSLEKVV--- 260

           +H+ H +   +E    +D  P V+S LP + P  H    + I T   SDL+ L K++   

Sbjct: 1139 FHNLHKFLGLKELCVRLDSKPDVLSVLPGEFPNLHHMEKLSIRTSTESDLSKLVKLIQNS 1198

Query: 261 ---HFYDDKVQSTYFLTRPEPHFTIVV----IFESKKSERDSHFISFLNEL 304

              H +  K     FL+  EP  T++     + E + S R    ++F+N L

Sbjct: 1199 PNLHVFHLKCN---FLSNCEPLMTVLASCKKLREIEFSGRCFEAMTFVNIL 1246

>PA177473.19|RalGDS-like factor\

         Length = 778

Score = 28.9 bits (63), Expect = 5.3
Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 9/59 (15%)

Query: 50 SSRFHHPI-----LSPLESSFQLEVGVLS----HLLKAQAQISEWKFLPSLVTLHNAHT 99

          +SR + P+     L P  SS +L  G L     HLL A+   ++  F P+L+  H A T

Sbjct: 70 TSRQYRPLDPLAPLPPPRSSRRLRAGTLEALVRHLLDARTAGADMMFTPALLATHRAFT 128

>PA82764.1|YY1 transcription factor\

         Length = 414

Score = 28.9 bits (63), Expect = 5.3
Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 2/49 (4%)

Query: 199 GSESFQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVXHWPNVIXIXT 247

          G     GH +HH H +   P  +   P V   P+   V H   VI + T

Sbjct: 64 GGHGHAGHHHHHHHHHHHHPPMIALQPLVTDDPTQ--VHHHQEVILVQT 110

 Database: RIKEN IPS7 Protein Sequences
   Posted date:  Jul 19, 2005  2:33 PM
 Number of letters in database: 15,064,402
 Number of sequences in database:  33,451
 

Lambda K H

  0.318    0.132    0.390 

Gapped Lambda K H

  0.267   0.0410    0.140 

Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,633,615 Number of Sequences: 33451 Number of extensions: 319325 Number of successful extensions: 769 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 764 Number of HSP's gapped (non-prelim): 6 length of query: 323 length of database: 15,064,402 effective HSP length: 101 effective length of query: 222 effective length of database: 11,685,851 effective search space: 2594258922 effective search space used: 2594258922 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 61 (28.1 bits)