2i2O Protein Evolution Presentation: Difference between revisions
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'''Figure 1.0''' Protein sequence of MIF4G domain containing protein found in Homo sapiens. | '''Figure 1.0''' Protein sequence of MIF4G domain containing protein found in Homo sapiens. | ||
[[Image:Alignment sequence 1.JPG|Description]] | |||
[[Image:alignment 2.JPG|600 px|Description]] | |||
[[Image:alignment 3.JPG|Description]] | |||
[[Image:alignment 4.JPG|Description]] | |||
'''Figure 1.1''' Multiple alignment sequence of the MIF4G domain containing protein. The top sequence is the human sequence of the MIF4G protein and the focus of this report. | |||
Revision as of 01:59, 12 June 2007
- Evolution of eIF4G occurred early on in euks
- Evolution of three domains within eIF4G - MIF4G being one of them
- Research indicates all three domains present in early euks
- Possible loss of these domains within other organisms
- Three programs used to construct phylogenetic tree
- Blast used to find significant sequences
- 54 sequences chosen because E-value under 0.01
- ClustalX used
- Multiple alignment of the 55 sequences undertaken
- First draft phlogenetic tree created
Figure 1.0 Protein sequence of MIF4G domain containing protein found in Homo sapiens.
Figure 1.1 Multiple alignment sequence of the MIF4G domain containing protein. The top sequence is the human sequence of the MIF4G protein and the focus of this report.
Figure 1.1 Phylogenetic tree of the 55 significant sequences closest to MIFG4 protein. Bootstrapping values shown on the tree. * denotes a bootstrap value less than 75
