3b5q Function: Difference between revisions

From MDWiki
Jump to navigationJump to search
No edit summary
 
(37 intermediate revisions by 2 users not shown)
Line 1: Line 1:
[[Image:ASK.png]]
Protein data base profile characteris arylsulfatase asa hydrolase and a sulfatase. A hydrolase is an enzyme which hydrolyses a chemical bond. The general reaction can be shown as follows.
Protein data base profile characteris arylsulfatase asa hydrolase and a sulfatase. A hydrolase is an enzyme which hydrolyses a chemical bond. The general reaction can be shown as follows.
[[Image:3b5q image PDB.png]]


[[Image:hydrolase.png]]
[[Image:hydrolase.png]]
Line 16: Line 22:
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as '''EC 3.1.6.''' in enzyme classifications. Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as '''EC 3.1.6.''' in enzyme classifications. Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.


Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB)( Also a lysosomal enzyme) hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate.
Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB),also a lysosomal enzyme, which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate.
 
 
Arylsulphatase C (ASD), E (ASE) and steryl-sulphatase (STS)
a membrane bound microsomal enzyme which hydrolyzes 3-beta-hydroxy steroid sulphates; iduronate 2-sulphatase precursor (IDS), a lysosomal enzyme that hydrolyzes the 2-sulphate groups from non-reducing-terminal iduronic acid residues in dermatan sulphate and heparan sulphate; N-acetylgalactosamine-6-sulphatase , which hydrolyzes the 6-sulphate groups of the N-acetyl-d-galactosamine 6-sulphate units of chondroitin sulphate and the D-galactose 6-sulphate units of keratan sulphate; glucosamine-6-sulphatase (G6S), which hydrolyzes the N-acetyl-D-glucosamine 6-sulphate units of heparan sulphate and keratan sulphate; N-sulphoglucosamine sulphohydrolase (sulphamidase), the lysosomal enzyme that catalyzes the hydrolysis of N-sulpho-d-glucosamine into glucosamine and sulphate; sea urchin embryo arylsulphatase ; green algae arylsulphatase , which plays an important role in the mineralization of sulphates; and arylsulphatase from Escherichia coli (aslA), Klebsiella aerogenes (gene atsA) and Pseudomonas aeruginosa (gene atsA).
Clan


==Functional site==
==Functional site==
MSA data revealed some conserved residues on the sequence. They were mapped on the three dimantional structure.
MSA data revealed some conserved residues on the sequence. They were mapped on the three dimentional structure.
 
[[Image:Zn_Cl_n_pocket.png|600px|thumb]]
 
 
Highly conserved regions of the protein revealed in MSA are highlighted in blue, while ligands; Zn and Cl; are shown in green and grey respectively. However region of high conservation seems to be physically further from ligand binding sites. May be sudgessting that ligands only play a role in stabilizing the protein conformation, hense the active site; for the enzyme to perfprm its catalytic functions.
 
 
[[Image:Zn_Cl_surface.png|600px|thumb]].
 
 
 
 
 
 
 
 
 
 
 






[[Image:ASAsite1.png]]






'''''Figure 1:''' Catalytic site of Arylsulfatase A (ASA) with the Magnesium ion bound. However H229 is only conserved within Arylsulfatase A of different species.




Line 55: Line 39:




[[Image:ASKsite1.png]]


'''''figure 2:''''' Catalytic site of Arylsulfatase K (ASK) with conserved catalytic residues. Residues marked in '''grey''' are possible to be involved in divalent metal binding , while residues marked in '''magenta''' are highly conserved with those of Arylsulfatase A (ASA) and steroid sulfatase (STS). Histidine (H) shown in '''cyan''' is not strictly consetved in '''MSA''', but the only available nucleophile in the close proximity.




Highly conserved regions of the protein revealed in MSA are highlighted in blue, while ligands; Zn and Cl; are shown in green and grey respectively. However, region of high conservation seems to be physically further from ligand binding sites. Maybe suggesting that ligands only play a role in stabilizing the protein conformation, hence the active site; for the enzyme to perform it's catalytic functions.




[[Image:Zn_Cl_surface.png|thumb]].


== Structural alignment ==


*Three dimentional structure of arylsulfatase was aligned with other available structures using DALI server [http://ekhidna.biocenter.helsinki.fi/dali_server/results/20080513-017-30c6150342a20dabd7c2488208032bb4 (structural alignment)]. Results are shown in 'figure 4'.


  No:  Chain  Z    rmsd lali nres  %id  Description
  '''1:  3b5q-A 73.6  0.0  464  464  100  MOLECULE: PUTATIVE SULFATASE YIDJ;'''                                 
  '''2:  3b5q-B 70.2  0.3  464  467  100  MOLECULE: PUTATIVE SULFATASE YIDJ;'''                                 
  3:  2qzu-A 35.1  2.5  375  465  25  MOLECULE: PUTATIVE SULFATASE YIDJ;                                 
  4:  1fsu  28.7  2.8  344  474  22  MOLECULE: N-ACETYLGALACTOSAMINE-4-SULFATASE;                       
  5:  1n2l-A 28.4  3.0  343  483  25  MOLECULE: ARYLSULFATASE A;                                         
  6:  1n2k-A 28.3  3.2  344  482  25  MOLECULE: ARYLSULFATASE A;                                         
  7:  1e3c-P 28.2  3.2  344  481  26  MOLECULE: ARYLSULFATASE A;                                         
  8:  1e33-P 28.2  3.1  344  480  25  MOLECULE: ARYLSULFATASE A;                                         
  9:  1e2s-P 28.2  3.1  343  481  25  MOLECULE: ARYLSULFATASE A;                                         
  10:  1e1z-P 28.1  3.2  344  481  26  MOLECULE: ARYLSULFATASE A;                                         
  11:  1auk  27.9  3.1  343  481  25  MOLECULE: ARYLSULFATASE A; 


Figure 4: Structurally related proteins. (No 1 and 2 are two chains of arylsulfatase K).






*The function of highly related proteins were found using [http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/GetResults.pl?source=profunc&user_id=bw28&code=091700 ProFunc]
 


:'''Putative Sulfatase YIDI''' hydrolyses sulfuric ester bonds of its substrate hence significant in metabolism.
:'''Arylsulfatase A''' has both sulfuric ester hydrolase and phosphoric monoester hydrolase activities.


=Arylsulfatase K (BT1596) Interactions with other proteins=


[[Image:net.png]]


'''Input Protein'''  
*BT1596 Putative sulfatase yidJ (481 aa) of ''Bacteroides thetaiotaomicron''.  


'''Predicted Functional Partners'''  
*BT3796: Putative secreted sulfatase ydeN (518 aa).  
*BT1595 Transcription termination factor rho (722 aa).  
*BT1597 Two-component system sensor histidine kinase (539 aa).  
*BT3057 N-acetylgalactosamine-6-sulfatase (508 aa).  
*BT1598 Putative two-component system sensor histidine (655 aa).  
*BT3101 N-sulphoglucosamine sulphohydrolase (455 aa).  
*BT3489 Arylsulfatase B {UniProtKB/TrEMBL-Q8A219} (458 aa).




Line 77: Line 97:




'''N-acetylgalactosamine-6-sulfatase''' cleaves the 6-sulfate groups of  N-acetyl-D-galactosamine 6-sulfate units in chondroitin sulfate and D-galactose 6-sulfate units in keratan sulfate.'''N-sulphoglucosamine sulphohydrolase''' is also known as heparine sulfamidase, which catalyses the hydrolysis of Sulfur-Nitrogen bonds. N-sulphoglucosamine sulphohydrolase is responsible for the degradation of glucosaminlglycan and glycan structure of extra cellular matrix.


:'''N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine + sulfate'''


N-acetylgalactosamine-4- sulfatase (Arylsulfatase B) hydrolyse the sulfate ester group from N-acetylgalactosamine 4-sulfate of dermatine sulfate. Deficiency of ASB cause a rare mucopolysaccharidosis (MPS IV; Maroteaux-Lamy syndrome)




Line 101: Line 124:




=Arylsulfatase K (BT1596) Interactions with other proteins=
[[Image:net.png|600px|thumb]]
'''Input Protein'''  
*BT1596 Putative sulfatase yidJ (481 aa) of ''Bacteroides thetaiotaomicron''.  
'''Predicted Functional Partners'''  
*BT3796: Putative secreted sulfatase ydeN (518 aa).  
*BT1595 Transcription termination factor rho (722 aa).  
*BT1597 Two-component system sensor histidine kinase (539 aa).  
*BT3057 N-acetylgalactosamine-6-sulfatase (508 aa).  
*BT1598 Putative two-component system sensor histidine (655 aa).  
*BT3101 N-sulphoglucosamine sulphohydrolase (455 aa).  
*BT3489 Arylsulfatase B {UniProtKB/TrEMBL-Q8A219} (458 aa).
'''N-acetylgalactosamine-6-sulfatase''' cleaves the 6-sulfate groups of  N-acetyl-D-galactosamine 6-sulfate units in chondroitin sulfate and D-galactose 6-sulfate units in keratan sulfate.'''N-sulphoglucosamine sulphohydrolase''' is also known as heparine sulfamidase, which catalyses the hydrolysis of Sulfur-Nitrogen bonds. N-sulphoglucosamine sulphohydrolase is rsponsible for the degradation of glucosaminlglycan and glycan structure of extra cellular matrix.
:'''N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine + sulfate'''
== Structural alignment ==
*Three dimentional structure of arylsulfatase was aligned with other available structures using DALI server [http://ekhidna.biocenter.helsinki.fi/dali_server/results/20080513-017-30c6150342a20dabd7c2488208032bb4 (structural alignment)]. Resuls are shown in 'figure 4'.
  No:  Chain  Z    rmsd lali nres  %id  Description
  '''1:  3b5q-A 73.6  0.0  464  464  100  MOLECULE: PUTATIVE SULFATASE YIDJ;'''                                 
  '''2:  3b5q-B 70.2  0.3  464  467  100  MOLECULE: PUTATIVE SULFATASE YIDJ;'''                                 
  3:  2qzu-A 35.1  2.5  375  465  25  MOLECULE: PUTATIVE SULFATASE YIDJ;                                 
  4:  1fsu  28.7  2.8  344  474  22  MOLECULE: N-ACETYLGALACTOSAMINE-4-SULFATASE;                       
  5:  1n2l-A 28.4  3.0  343  483  25  MOLECULE: ARYLSULFATASE A;                                         
  6:  1n2k-A 28.3  3.2  344  482  25  MOLECULE: ARYLSULFATASE A;                                         
  7:  1e3c-P 28.2  3.2  344  481  26  MOLECULE: ARYLSULFATASE A;                                         
  8:  1e33-P 28.2  3.1  344  480  25  MOLECULE: ARYLSULFATASE A;                                         
  9:  1e2s-P 28.2  3.1  343  481  25  MOLECULE: ARYLSULFATASE A;                                         
  10:  1e1z-P 28.1  3.2  344  481  26  MOLECULE: ARYLSULFATASE A;                                         
  11:  1auk  27.9  3.1  343  481  25  MOLECULE: ARYLSULFATASE A; 
Figure 4: Structurally related proteins. (No 1 and 2 are two chains of arylsulfatase K).
*The function of highly related proteins were found using [http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/GetResults.pl?source=profunc&user_id=bw28&code=091700 ProFunc]
 
:'''Putative Sulfatase YIDI''' hydrolyses sulfuric ester bonds of its substrate hence significant in metabolism.
:'''Arylsulfatase A''' has both sulfuric ester hydrolase and phosphoric monoester hydrolase activities.






== Comparison of Motifs ==
== Comparison of Motifs ==


=== 1 Putative sulfatase yidj motifs ===
=== 1 Putative sulfatase yidj motifs ===
Line 179: Line 142:




Motifs '''PF00884''' and '''PTHR10342''' are conserved among all three proteins. This is indicative of a some structural similarity in their substrates. These proteins may carry out a similar catalytic reaction or participate in slightly different aspects of a common catalytic activity. The second possibility seems to be stronger considering  all three enzymes are found in tha same metabolic pathway, in close proximity to each other. All the resulted motifs are found in a '''sulfatase enzyme''' while they all belong to '''Alkaline phosphatase-like''' protein superfamily.   
Motifs '''PF00884''' and '''PTHR10342''' are conserved among all three proteins. This is indicative of a some structural similarity in their substrates. These proteins may carry out a similar catalytic reaction or participate in slightly different aspects of a common catalytic activity. The second possibility seems to be stronger considering  all three enzymes are found in tha same metabolic pathway, in close proximity to each other. All the resulted motifs are found in a '''sulfatase enzyme''' while they all belong to '''Alkaline phosphatase-like''' protein superfamily.
 
'''PTHR10342''' is responsible for synthesis and breakdown of sulfur containing compounds, and sulfur-dependent redox reactions.   
 





Latest revision as of 11:33, 6 June 2008

ASK.png

Protein data base profile characteris arylsulfatase asa hydrolase and a sulfatase. A hydrolase is an enzyme which hydrolyses a chemical bond. The general reaction can be shown as follows.


3b5q image PDB.png

Hydrolase.png


Hydrolases are named as EC 3 in enzyme classification.




Function of sulfatases

Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as EC 3.1.6. in enzyme classifications. Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.

Arylsulphatase A (ASA) is a lysosomal enzyme which hydrolyzes cerebroside sulphate. Arylsulphatase B (ASB),also a lysosomal enzyme, which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatan sulphate.

Functional site

MSA data revealed some conserved residues on the sequence. They were mapped on the three dimentional structure.


ASAsite1.png


Figure 1: Catalytic site of Arylsulfatase A (ASA) with the Magnesium ion bound. However H229 is only conserved within Arylsulfatase A of different species.



ASKsite1.png

figure 2: Catalytic site of Arylsulfatase K (ASK) with conserved catalytic residues. Residues marked in grey are possible to be involved in divalent metal binding , while residues marked in magenta are highly conserved with those of Arylsulfatase A (ASA) and steroid sulfatase (STS). Histidine (H) shown in cyan is not strictly consetved in MSA, but the only available nucleophile in the close proximity.


Highly conserved regions of the protein revealed in MSA are highlighted in blue, while ligands; Zn and Cl; are shown in green and grey respectively. However, region of high conservation seems to be physically further from ligand binding sites. Maybe suggesting that ligands only play a role in stabilizing the protein conformation, hence the active site; for the enzyme to perform it's catalytic functions.


Zn Cl surface.png

.

Structural alignment

  • Three dimentional structure of arylsulfatase was aligned with other available structures using DALI server (structural alignment). Results are shown in 'figure 4'.
 No:  Chain   Z    rmsd lali nres  %id   Description
  1:  3b5q-A 73.6  0.0  464   464  100   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  2:  3b5q-B 70.2  0.3  464   467  100   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  3:  2qzu-A 35.1  2.5  375   465   25   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  4:  1fsu   28.7  2.8  344   474   22   MOLECULE: N-ACETYLGALACTOSAMINE-4-SULFATASE;                         
  5:  1n2l-A 28.4  3.0  343   483   25   MOLECULE: ARYLSULFATASE A;                                           
  6:  1n2k-A 28.3  3.2  344   482   25   MOLECULE: ARYLSULFATASE A;                                           
  7:  1e3c-P 28.2  3.2  344   481   26   MOLECULE: ARYLSULFATASE A;                                           
  8:  1e33-P 28.2  3.1  344   480   25   MOLECULE: ARYLSULFATASE A;                                           
  9:  1e2s-P 28.2  3.1  343   481   25   MOLECULE: ARYLSULFATASE A;                                           
 10:  1e1z-P 28.1  3.2  344   481   26   MOLECULE: ARYLSULFATASE A;                                           
 11:  1auk   27.9  3.1  343   481   25   MOLECULE: ARYLSULFATASE A;  

Figure 4: Structurally related proteins. (No 1 and 2 are two chains of arylsulfatase K).


  • The function of highly related proteins were found using ProFunc


Putative Sulfatase YIDI hydrolyses sulfuric ester bonds of its substrate hence significant in metabolism.
Arylsulfatase A has both sulfuric ester hydrolase and phosphoric monoester hydrolase activities.

Arylsulfatase K (BT1596) Interactions with other proteins

Net.png


Input Protein

  • BT1596 Putative sulfatase yidJ (481 aa) of Bacteroides thetaiotaomicron.

Predicted Functional Partners

  • BT3796: Putative secreted sulfatase ydeN (518 aa).
  • BT1595 Transcription termination factor rho (722 aa).
  • BT1597 Two-component system sensor histidine kinase (539 aa).
  • BT3057 N-acetylgalactosamine-6-sulfatase (508 aa).
  • BT1598 Putative two-component system sensor histidine (655 aa).
  • BT3101 N-sulphoglucosamine sulphohydrolase (455 aa).
  • BT3489 Arylsulfatase B {UniProtKB/TrEMBL-Q8A219} (458 aa).



N-acetylgalactosamine-6-sulfatase cleaves the 6-sulfate groups of N-acetyl-D-galactosamine 6-sulfate units in chondroitin sulfate and D-galactose 6-sulfate units in keratan sulfate.N-sulphoglucosamine sulphohydrolase is also known as heparine sulfamidase, which catalyses the hydrolysis of Sulfur-Nitrogen bonds. N-sulphoglucosamine sulphohydrolase is responsible for the degradation of glucosaminlglycan and glycan structure of extra cellular matrix.

N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine + sulfate

N-acetylgalactosamine-4- sulfatase (Arylsulfatase B) hydrolyse the sulfate ester group from N-acetylgalactosamine 4-sulfate of dermatine sulfate. Deficiency of ASB cause a rare mucopolysaccharidosis (MPS IV; Maroteaux-Lamy syndrome)













Comparison of Motifs

1 Putative sulfatase yidj motifs

Bw28.jpg

2 Arylsulfatase A. Synonym: cerebroside-3-sulfate-sulfatase motifs

1auk.jpg

3 N-acetylgalactosamine-4-sulfatase motifs. (Chain: a. Synonym: arylsulfatase b, asb, 4-sulfatase)

1fsu.jpg


Motifs PF00884 and PTHR10342 are conserved among all three proteins. This is indicative of a some structural similarity in their substrates. These proteins may carry out a similar catalytic reaction or participate in slightly different aspects of a common catalytic activity. The second possibility seems to be stronger considering all three enzymes are found in tha same metabolic pathway, in close proximity to each other. All the resulted motifs are found in a sulfatase enzyme while they all belong to Alkaline phosphatase-like protein superfamily.

PTHR10342 is responsible for synthesis and breakdown of sulfur containing compounds, and sulfur-dependent redox reactions.

































Click here to go Back