3b5q Function

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Protein data base profile characteris arylsulfatase asa hydrolase and a sulfatase. A hydrolase is an enzyme which hydrolyses a chemical bond. The general reaction can be shown as follows.

Hydrolase.png


Group of hydrolaes are named as EC 3 in enzyme classification.




== 1. Function of sulfatases ==

Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. These are categorised as EC 3.1.6. in enzyme classifications. Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.

== 2. Functional site== MSA data revealed some conserved residues on the sequence. They were mapped on the three dimantional structure.

Zn Cl n pocket.png


Highly conserved regions of the protein revealed in MSA are highlighted in blue, while ligands; Zn and Cl; are shown in green and grey respectively. However region of high conservation seems to be physically further from ligand binding sites. May be sudgessting that ligands only play a role in stabilizing the protein conformation, hense the active site; for the enzyme to perfprm its catalytic functions.

Zn Cl surface.png

.




== 3. Arylsulfatase K (BT1596) Interactions with other proteins==

Net.png


Input Protein

  • BT1596 Putative sulfatase yidJ (481 aa) of Bacteroides thetaiotaomicron.

Predicted Functional Partners

  • BT3796: Putative secreted sulfatase ydeN (518 aa).
  • BT1595 Transcription termination factor rho (722 aa).
  • BT1597 Two-component system sensor histidine kinase (539 aa).
  • BT3057 N-acetylgalactosamine-6-sulfatase (508 aa).
  • BT1598 Putative two-component system sensor histidine (655 aa).
  • BT3101 N-sulphoglucosamine sulphohydrolase (455 aa).
  • BT3489 Arylsulfatase B {UniProtKB/TrEMBL-Q8A219} (458 aa).

N-acetylgalactosamine-6-sulfatase cleaves the 6-sulfate groups of N-acetyl-D-galactosamine 6-sulfate units in chondroitin sulfate and D-galactose 6-sulfate units in keratan sulfate.N-sulphoglucosamine sulphohydrolase is also known as heparine sulfamidase, which catalyses the hydrolysis of Sulfur-Nitrogen bonds. N-sulphoglucosamine sulphohydrolase is rsponsible for the degradation of glucosaminlglycan and glycan structure of extra cellular matrix.

N-sulfo-D-glucosamine + H(2)O <=> D-glucosamine + sulfate






























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