ATP binding domain 4 Structures: Difference between revisions

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[[Image:CAST-2nz2.PNG|right|thumb|500px|'''Figure 13'''. The topology of 2NZ2 generated via CASTp]]
[[Image:CAST-2nz2.PNG|right|thumb|500px|'''Figure 13'''. The topology of 2NZ2 generated via CASTp]]
[[Image:CAST-3bl5.PNG|left|thumb|500px|'''Figure 14'''. The topology of 3BL5 generated via CASTp]]
[[Image:CAST-3bl5.PNG|left|thumb|500px|'''Figure 14'''. The topology of 3BL5 generated via CASTp]]
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The volume and size of the cleft of between protein indicated similarity between 1RU8 and 2NZ2. While 3BL5 less similarity when compared to both 1RU8 and 2NZ2

Revision as of 12:54, 2 June 2009

General Properties

General information from PDB indicates that :

(a) 1RU8 is a putative n-type ATP pyrophosphatase isolated from Pyrococcus furiosus, expressed in Escherichia Coli.

(b) Is a member of clan PP-loop

(c) Resolution of 2.7 angstroms, with an r-value of 0.218.

(d) Ligand chemical component identified as TRS (2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL).

Surface Structure

Pymol Visualization

Figure 1. The three-dimensional structure of secondary structure 1RU8, as generated by PyMOL

Purple- Helix
Yellow - Sheet
Green- Loop


Figure 2.0. The three-dimensional surface structure of 1RU8, as generated by PyMOL. Note that there is a artificial ligand, TRS (purple) bound to the protein
Figure 2.1. 1RU8 indicates its a dimer























































Secondary Structure and Location of PP-loop

Figure 4. Conserved residues of 1RU8 via PDBsum
Figure 5. Conserved residues of 1RU8 based on figure 4 visualized in Pymol. Highlighted red is the really conserved region and highlighted blue is the residues that is conserved and interact with the ligand



































Electrostatic Surface Potential

Figure 7. The electrostatic surface potential. Blue colour indicated positive charge and red colour indicated negative charge surface whereas white colour indicated neutral charge

Structure Similarities

Figure 8. The list of structural similarities to 1RU8 obtained using DALI. Highlighted by the box is the most similar structure that has known function which are used in this investigation.

Z score , the statistical significance of the similarity between protein-of-interest and other neighbourhood proteins. The program optimizes a weighted sum of similarities of intramolecular distances.

Root Mean Square Distance (RMSD), root-mean-square deviation of C-alpha atoms in the least-squares superimposition of the structurally equivalent C-alpha atoms. RMSD is not optimized and is only reported for information.

lali, the number of structurally equivalent residues.

nres, or the total number of amino acids in the hit protein.

%id, percentage of identical amino acids over structurally equivalent residues.

Structure Alignment

1RU8 and 2NZ2

Figure 9.0. Structure alignment between 1RU8 and 2NZ2 generated via PyMol-Front view
Figure 9.1. Structure alignment between 1RU8 and 2NZ2 generated via PyMol-Back view

Highlighted in green is the 2NZ2 while in magenta is the 1RU8. Since 1RU8 has 2 domain, domain B is coloured darker in magenta for differentiation. There are 2 ligand indicated by the yellow (Citrulline) and red (ATP) spheres from 2NZ2. Highlighted also is the conserved region of PP-loop in pink and blue for 1RU8 and 2NZ2 respectively.


1RU8 and 3BL5

Figure 10. Structure alignment between 1RU8 and 3BL5 generated via PyMol




















Surface Cleft and Topography

Figure 12. The topology of 1RU8 generated via CASTp
Figure 13. The topology of 2NZ2 generated via CASTp
Figure 14. The topology of 3BL5 generated via CASTp

























The volume and size of the cleft of between protein indicated similarity between 1RU8 and 2NZ2. While 3BL5 less similarity when compared to both 1RU8 and 2NZ2