Arylformamidase Discussion: Difference between revisions
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probable function | probable function | ||
The carboxylesterase 2c7b was chosen for further analysis due to its high structural similarity to 2pbl. 2c7b is a thermostable carboxylesterase from an uncultured archaeon which was isolated in a thermal environmental sample (Byun 2007). This carboxylesterase belongs to a family of bacterial hormone-sensitive lipases/esterases (HSLs) known for their amino-acid sequence similarity to the mammalian hormone-sensitive lipase (ARPIGNY 1999). There were a number of other bacterial HSL proteins found to have a similar structure to 2pbl. Although in all cases there was a relatively low sequence similarity with 2pbl, regions characteristic to HSL proteins were found to be conserved indicating that 2pbl may indeed be another bacterial HSL (Arpigny 1999, see figure…). | |||
The function of 2c7b has been well characterised (references). | The function of 2c7b has been well characterised (references). | ||
Revision as of 08:22, 3 June 2008
probable function
The carboxylesterase 2c7b was chosen for further analysis due to its high structural similarity to 2pbl. 2c7b is a thermostable carboxylesterase from an uncultured archaeon which was isolated in a thermal environmental sample (Byun 2007). This carboxylesterase belongs to a family of bacterial hormone-sensitive lipases/esterases (HSLs) known for their amino-acid sequence similarity to the mammalian hormone-sensitive lipase (ARPIGNY 1999). There were a number of other bacterial HSL proteins found to have a similar structure to 2pbl. Although in all cases there was a relatively low sequence similarity with 2pbl, regions characteristic to HSL proteins were found to be conserved indicating that 2pbl may indeed be another bacterial HSL (Arpigny 1999, see figure…).
The function of 2c7b has been well characterised (references).
additional info on probable function
Carboxylesterases have a common reaction mechanism (see figure ...). This is somewhat similar to the arylformamidase reaction mechanism incorporating hydrolysis of a ... bond.
evolutionary link...
biological implications/applications...
Potential thermostability - applicability in industry.
further research...
