Arylformamidase Function Slide 8: Difference between revisions

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== Thermostable? ==
== Thermostable? ==


- Byun, et al. (2007) identified a number of HSL family members with thermostable properties.
- A number of these HSLs identified by Byun, et al. 2007 had thermostable properties.


[[Image:DALI2pbl.png|centre|framed|'''Thermophillic HSL family members as identified by Byun, et al. 2007 found in the DALI search performed by Basma.''']]
[[Image:DALI2pbl.png|centre|framed|'''Thermophillic HSL family members as identified by Byun, et al. 2007 found in the DALI search performed by Basma.''']]


- Similar sequence in regions characteristic to this family of proteins (Arpigny and Jaeger 1999).
- Is 2pbl thermostable as well?
 
- Similar structure and sequence.


- '''Ecological evidence:''' Weak - an aquatic organism widely present throughout world's oceans.
- '''Ecological evidence:''' Weak - an aquatic organism widely present throughout world's oceans.


== The Putative Function Refined ==
- Evidence points to monomer not dimer.
 
Exact function difficult to specify.
 
Broad range of functions performed by HSLs.
 
May be a thermostable carboxylesterase.  


Applications?
- Experimentation needed.


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Latest revision as of 06:39, 10 June 2008

Thermostable?

- A number of these HSLs identified by Byun, et al. 2007 had thermostable properties.

Thermophillic HSL family members as identified by Byun, et al. 2007 found in the DALI search performed by Basma.

- Is 2pbl thermostable as well?

- Ecological evidence: Weak - an aquatic organism widely present throughout world's oceans.

- Evidence points to monomer not dimer.

- Experimentation needed.

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