Arylformamidase Methods: Difference between revisions
From MDWiki
Jump to navigationJump to search
Thomasparker (talk | contribs) No edit summary |
Thomasparker (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
'''Literature search''' | |||
A literature search was performed using the putative annotation ‘arylformamidase’. A paper by Pabarcus et al. 2007 was returned which, ironically, described the arylformamidase from Mus Musculus. | |||
'''Conservation of Catalytic Triad''' | |||
An alignment of 2pbl and the protein of interest was performed using ClustalX. Default parameters were used. Residues of the catalytic triad were identified from the paper describing it and located in the sequence. Conservation of the residue and the surrounding sequence was observed. Note: in analysing conservation of the 2c7b catalytic triad with 2pbl, the clustalW alignment was found to differ from the alignment provided as part of the DALI results. | |||
[[Arylformamidase | Return to the main page...]] | [[Arylformamidase | Return to the main page...]] | ||
Revision as of 05:03, 3 June 2008
Literature search
A literature search was performed using the putative annotation ‘arylformamidase’. A paper by Pabarcus et al. 2007 was returned which, ironically, described the arylformamidase from Mus Musculus.
Conservation of Catalytic Triad
An alignment of 2pbl and the protein of interest was performed using ClustalX. Default parameters were used. Residues of the catalytic triad were identified from the paper describing it and located in the sequence. Conservation of the residue and the surrounding sequence was observed. Note: in analysing conservation of the 2c7b catalytic triad with 2pbl, the clustalW alignment was found to differ from the alignment provided as part of the DALI results.
