Arylformamidase Sequence & Homology

Method

Using the query sequence pdb:2pbl "Arylformamidase", a BLAST search was performed using a non-redundant database. The top scoring matches to an E-value of 3e-054, 35 sequences in total, were selected. Eukaryotic homologous sequences sequences were found using HOMOLOGENE. These were appended to the list and a multple sequece alignment was performed using CLUSTAL X.

The data output from the multiple sequence alignment was bootstrapped 100 times and a phylogenetic tree was created using the neighbour-joining method. The program FigTree was used to create the visual representation of this tree(Figure 1).

Figure 1.

Unrooted phylogenetic tree of highest scoring results from a BLAST search of bacterial sequnces using a non-redundant database and homologous eukaryotic sequences sourced from HOMOLOGENE:

Results

The alignment revealed several conserved regions accross all species, thereby indicating a high level of conservation from Bacteria through Eukaryota. These included vertebrates, invertebrates, plants and single-celled eukaryotes.