Arylformamidase Structure

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1. Structure of Arylformamidase

Arylformamidase


Whole protein.png

The image above shows the chains A (upper right), B (upper left), C (lower right) & D (lower left) interacting. The molecules in the middle of chains A & B and chains C & D is phosphate ion (PO4). The green molecule between chain B & D is a magnesium ion (Mg). These ions aren't biologically significant and could only be an artefact. Those chains exist as indivitual functional units.



Chain A of arylformamidase


Chain A.jpg

The red molecule in the middle is an unknown ligand containing a ring composed of 9 oxygen molecules. The green sphere is a chloride ion.

2. Putative Thioesterase

Thioesterases split ester groups into acid and alcohol in the presence of water, specifically at a thiol group.

Thioesterases or thiolester hydrolases are members of E.C.3.1.2.

Our structure resembles carboxylesterases (hydrolases) hence it is predicted that it has similar cellular activity.



3. Interaction of human arylformamidase (AFMID) with other proteins

Confidence interaction with names.png


The interaction between the proteins have been determined from curated STRING database (significant score). However there is no significant evidence for:

1- Neighborhood in the genome

2- Gene fusions

3- Cooccurence across genomes

4- Co-Expression

5- Experimental/Biochemical data


4. Interaction of Silicibacter Sp. arylformamidase (AFMID) with other proteins

Examplec.jpg


TM1040_2226 Tryptophan 2,3-dioxygenase (279 aa)

TM1040_2225 Kynureninase (396 aa)

TM1040_2493 Succinic semialdehyde dehydrogenase (490 aa)

TM1040_1862 Hypothetical protein (212 aa)

TM1040_2491 Creatinase (402 aa)

TM1040_2736 Transketolase, putative (794 aa)

4. DALI OUTPUT

File:DALI RESULT.txt



Metagenomic Archea Carboxylesterase A chain

Carboxylase A chain.PNG

File:Carboxylase.txt

PDB link title


Archaeoglobus fulgidus Carboxylesterase (Chains A B C D)

Archeaon Carboxylesterase.PNG

File:Carboxylesterase (archaeon).txt

PDB link title



5. Secondary structure analysis

PDBSum output for arylformamidase

PDBSum pblA.PNG

PDBSUM [1]


Archeon Carboxylesterase secondary structure

PDBsums.png



6. Topology

Topology.gif

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