C1orf41 Methods: Difference between revisions
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The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI). The sequence was used to find homologues of this protein using Blastp non-redundant protein database. Sequences of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed weak poor alignment were discarded from further investigation. From the sequence alignment, an evolutionary tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates. | The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI). The sequence was used to find homologues of this protein using Blastp non-redundant protein database. Sequences of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed weak poor alignment were discarded from further investigation. From the sequence alignment, an evolutionary tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates. | ||
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Revision as of 01:08, 9 June 2009
The sequence of the c1orf41 was obtained from National Center for Biotechnology Information (NCBI). The sequence was used to find homologues of this protein using Blastp non-redundant protein database. Sequences of homolgues with E-value of less than 1e-16 was retrieved and aligned using Clustalx to find conserved regions. Homologues which showed weak poor alignment were discarded from further investigation. From the sequence alignment, an evolutionary tree was constructed to observe the evolutionary relationship of this protein with its homologues. The ensure the reliability of the evolutionary tree, bootstrap was performed with 100 replicates.
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