Chromosome 1 open reading frame 41 Evolution: Difference between revisions

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[[Image:BLAST.png]]
[[Image:BLAST.png]]
The above shows the result from BLASTP search. The sequences of these proteins are aligned to observe the conserved regions.


== ClustalX ==
== ClustalX ==
Multiple sequence alignment with all of the 27 sequences obtained from BLAST is shown below:


[[Image:Platypus.png]]
[[Image:Platypus.png]]
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[[Image:Msa16.png]]
[[Image:Msafinal.png]]
[[Image:Msa17.png]]
 
The residues involved in binding site were highlighted. However, these residues were only conserved in terrestrial eukaryotes and some marine eukaryotes. Changes in the conserved amino acids may lead to higher binding affinity to ligand or different folding of the protein leading to different function.




== Phylogenetic Tree ==
== Phylogenetic Tree ==
After obtaining multiple sequence alignment, phylogenetic tree was construct based on the result from multiple sequence alignment. Bootstrap values are shown on each branch.


[[Image:Treecolour.png]]
[[Image:Treecolour.png]]
The unrooted tree above gave information on the evolutionary relationship between different organisms. This protein seems to have evolved from marine eukaryotes (red) to amphibians (blue) and then to terrestrial eukaryotes (green).


[[Image:Tree.png]]
[[Image:Tree.png]]
The bootstrap value shows the reliability of the branch. Higher bootstrap value show higher reliability.




[[Chromosome 1 open reading frame 41|Back to main page]]
[[Chromosome 1 open reading frame 41|Back to main page]]

Revision as of 11:19, 11 June 2009

Blast

BLAST.png

ClustalX

Platypus.png


The sequence of platypus, urchin_156 and urchin_159 was removed due to poor alignment. Besides that, the sequence for g.alga_fap was removed as it was a subsequence of g.alga_itp25.


Msa16.png Msa17.png


Phylogenetic Tree

Treecolour.png

Tree.png


Back to main page

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