Chromosome 1 open reading frame 41 Evolution: Difference between revisions

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== Blast ==
[[Image:BLAST.png|centre|framed|'''Figure 1:''' BLAST result]]


[[Image:BLAST.png]]


The above shows the result from BLASTP search. The sequences of these proteins are aligned to observe the conserved regions.
H-(2x)-W-(29x)-R-(2x)-S-(2x)-E residues critical for galactose binding site


== ClustalX ==


Multiple sequence alignment with all of the 27 sequences obtained from BLAST is shown below:
[[Image:Msa16.png|centre|framed|'''Figure 2:''' Conserved region of alpha B domain.]]




[[Image:Platypus.png]]
[[Image:Msa17.png|centre|framed|'''Figure 3:''' Residues involved in binding of Ca2+ and possible binding site.]]




The sequence of platypus, urchin_156 and urchin_159 was removed due to poor alignment. Besides that, the sequence for g.alga_fap was removed as it was a subsequence of g.alga_itp25.  
[[Image:Treecolour.png|centre|framed|'''Figure 4:''' Phylogenetic tree of c1orf41 with its homologues. Red is marine eukaryote, blue is amphibian and green is terrestrial eukaryotes.]]




[[Image:Tree.png|centre|framed|'''Figure 5:''' Phylogenetic tree with bootstrap values]]


[[Image:Msafinal.png]]


== Phylogenetic Tree ==
[[Chromosome 1 open reading frame 41 Function|Next]]
 
After obtaining the multiple sequence alignment, phylogenetic tree was construct based on the result from multiple sequence alignment. Bootstrap values are shown on each branch.
 
[[Image:Tree.bmp]]

Latest revision as of 06:26, 16 June 2009

Figure 1: BLAST result


H-(2x)-W-(29x)-R-(2x)-S-(2x)-E residues critical for galactose binding site


Figure 2: Conserved region of alpha B domain.


Figure 3: Residues involved in binding of Ca2+ and possible binding site.


Figure 4: Phylogenetic tree of c1orf41 with its homologues. Red is marine eukaryote, blue is amphibian and green is terrestrial eukaryotes.


Figure 5: Phylogenetic tree with bootstrap values


Next