Chromosome 1 open reading frame 41 Evolution

Blast

The above shows the result from BLASTP search. The sequences of these proteins are aligned to observe the conserved regions.

ClustalX

Multiple sequence alignment with all of the 27 sequences obtained from BLAST is shown below:

The sequence of platypus, urchin_156 and urchin_159 was removed due to poor alignment. Besides that, the sequence for g.alga_fap was removed as it was a subsequence of g.alga_itp25.

The residues involved in binding site were highlighted. However, these residues were only conserved in terrestrial eukaryotes and some marine eukaryotes. Changes in the conserved amino acids may lead to higher binding affinity to ligand or different folding of the protein leading to different function.

Phylogenetic Tree

After obtaining multiple sequence alignment, phylogenetic tree was construct based on the result from multiple sequence alignment. Bootstrap values are shown on each branch.

The unrooted tree above gave information on the evolutionary relationship between different organisms. This protein seems to have evolved from marine eukaryotes (red) to (blue) and then to terrestrial eukaryotes (green).