DnaE Polymerase

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protein sequence E.coli K12

--ThomasHuber 09:38, 19 February 2008 (EST)

Polymerase III

--Brendan 10:39, 19 February 2008 (EST)

Comparative Analysis of Eubacterial DNA Polymerase III Alpha Subunits

--Benjamin 09:22, 26 February 2008 (EST)

Introduction to Polymerase III

Pol III core is a 1:1:1 heterotrimer of the α polymerase, ε 3'-5' proofreading exonuclease, and θ subunits (39,40). The α subunit (encoded by dnaE) contains the DNA polymerase activity, incorporating 8 nucleotides per second (ntd/s) similar to Pol III core (20 ntds/s)

The replisome is composed of the following:

1) 2 DNA Pol III enzymes, made up of α, ε and θ subunits.

  o the α subunit has polymerization activity.
  o the ε subunit has proofreading activity.
  o the θ subunit stimulates the ε subunit's proofreading.

2) 2 β units which act as sliding DNA clamps, they keep the polymerase bound to the DNA.

3) 2 τ units which connect the 2 DNA Pol III enzymes.

4) 1 γ unit which acts as a clamp loader for the lagging strand Okazaki fragments, helping the two β subunits to form a unit and bind to DNA. The γ unit is made up of 5 γ subunits.