FUNCTIONAL ANALYSIS: Difference between revisions
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During the ProFunc analysis [http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/runprog.pl?prog=funchtml&html_name=seq001_aln.html&pdbcode=ay99&user_id=&pdb_type=PROFUNC&code=053949 UniProt] was used to perform a multiple sequence alignment on our protein sequence. The top 2 results were both highly conserved as well as also being YlqF proteins. The rest of the 10 proteins listed were all either GTPase or GTP binding proteins. This sequence search supports the literature in its claim that the YlqF protein is required for the late step of 50s ribosomal subunit assembly. | During the ProFunc analysis [http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/runprog.pl?prog=funchtml&html_name=seq001_aln.html&pdbcode=ay99&user_id=&pdb_type=PROFUNC&code=053949 UniProt] was used to perform a multiple sequence alignment on our protein sequence. The top 2 results were both highly conserved as well as also being YlqF proteins. The rest of the 10 proteins listed were all either GTPase or GTP binding proteins. This sequence search supports the literature in its claim that the YlqF protein is required for the late step of 50s ribosomal subunit assembly. | ||
<pre>Aligned sequences: | |||
%-tage | |||
Seq. id. identity Name | |||
-------- -------- ---- | |||
Query - - Query sequence | |||
O31743 95.6 - O31743 YlqF protein. | |||
Q65JP4 84.6 - Q65JP4 YlqF (GTP-binding domain protein). | |||
Q845L2 70.1 - Q845L2 Hypothetical protein. | |||
Q9Z9S1 59.9 - Q9Z9S1 YlqF (BH2476 protein). | |||
Q4MFY1 65.9 - Q4MFY1 Hypothetical protein. | |||
Q5WFP0 53.5 - Q5WFP0 GTPase. | |||
Q5HPU7 50.9 - Q5HPU7 GTP-binding protein, putative. | |||
Q3XZB6 52.4 - Q3XZB6 GTP-binding protein. | |||
A0Q0X8 49.3 - A0Q0X8 GTP-binding protein, putative. | |||
Q03KX8 47.6 - Q03KX8 Predicted GTPase.</pre> | |||
[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/runprog.pl?prog=funchtml&html_name=seq001_aln.html&pdbcode=ay99&user_id=&pdb_type=PROFUNC&code=053949 ProFunc] | [http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/runprog.pl?prog=funchtml&html_name=seq001_aln.html&pdbcode=ay99&user_id=&pdb_type=PROFUNC&code=053949 ProFunc] | ||
Revision as of 12:32, 10 June 2007
50S ribosome assembly in Bacillus subtilis
Literature supporting conclusion
During the ProFunc analysis UniProt was used to perform a multiple sequence alignment on our protein sequence. The top 2 results were both highly conserved as well as also being YlqF proteins. The rest of the 10 proteins listed were all either GTPase or GTP binding proteins. This sequence search supports the literature in its claim that the YlqF protein is required for the late step of 50s ribosomal subunit assembly.
Aligned sequences:
%-tage
Seq. id. identity Name
-------- -------- ----
Query - - Query sequence
O31743 95.6 - O31743 YlqF protein.
Q65JP4 84.6 - Q65JP4 YlqF (GTP-binding domain protein).
Q845L2 70.1 - Q845L2 Hypothetical protein.
Q9Z9S1 59.9 - Q9Z9S1 YlqF (BH2476 protein).
Q4MFY1 65.9 - Q4MFY1 Hypothetical protein.
Q5WFP0 53.5 - Q5WFP0 GTPase.
Q5HPU7 50.9 - Q5HPU7 GTP-binding protein, putative.
Q3XZB6 52.4 - Q3XZB6 GTP-binding protein.
A0Q0X8 49.3 - A0Q0X8 GTP-binding protein, putative.
Q03KX8 47.6 - Q03KX8 Predicted GTPase.
