Fascin Structure

Fascin Structure

Initially, analysis was done on the dimeric form of Fascin used for crystal structure analysis. Consultation with Ali Malik, who was dealing with function of the protein uncovered the fact that this protein actually works as a monomer. Subsequent visual analysis of the sequence for chain A and B from the crystal structure showed they were in fact identical and it is likely that in vitro crystalization required dimerism of the molecule. After this was established, I modified the pdb to represent only the original A chain and redid analysis with the monomeric (active form) of the protein.

References:

Joe Dundas, Zheng Ouyang, Jeffery Tseng, Andrew Binkowski, Yaron Turpaz, and Jie Liang 2006. CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated resiudes.Nucleic Acid Research, 34:W116-W118.

   *   Dolinsky TJ, Czodrowski P, Li H, Nielsen JE, Jensen JH, Klebe G, Baker NA. PDB2PQR: Expanding and upgrading automated preparation of biomolecular structures for molecular simulations. Nucleic Acids Res, 35, W522-5, 2007.
   * Dolinsky TJ, Nielsen JE, McCammon JA, Baker NA. PDB2PQR: an automated pipeline for the setup, execution, and analysis of Poisson-Boltzmann electrostatics calculations. Nucleic Acids Res, 32, W665-W667 (2004).