File:Untitled.bmp

METHODS

The protein that is used in this research is a putative n-type ATP pyrophosphatase from Pyrococcus furiosus. This protein is predicted to be similar to ATP binding domain 4. From ‘Target Blast and Symatlas Table’, a Blastp search against the NCBI non-redundant protein database can be conducted for both sequences from human and mouse ATP binding domain 4. Sequences with small E value were selected and Fasta format of selected sequences were retrieved. Using these sequences, a multiple sequence alignment was conducted by using ClustalX. A phylogenetic tree can be constructed based on the best multiple sequence alignment so that the evolutionary relationship between species can be observed.

RESULTS

From the Blastp search, it was found that the E value for both query sequence i.e; 1ru8A and the human ortholog, are very low. The highest E-value for 1ru8A and the human ortholog are 2e-25 and 1e-49 respectively which are very low. Therefore, all 200 sequences were aligned by using ClustalX. From the multiple sequence alignment, it was found that only one conserved region presence in the sequences. Therefore, only extremely low E values were re-selected and unrelated sequences were ignored and deleted. Based on these new re-selected sequences, it was found that there are 7 conserved residues.