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The predicted function based on the evolution and structure
The predicted function based on the evolution and structure
<font size = "4">'''Hydrolase'''</font>




[[Image:Document18_01.png]]
[[Image:Document18_01.png]]


'''Hydrolyase catalyze the hydrosis of the chemical bond between A and B, resulting of 2 simple molecules
Hydrolyase catalyze the hydrosis of the chemical bond between A and B, resulting of 2 simple molecules




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<font size = "4">'''Pfam'''</font>
{|cellspacing="0" cellpadding = "10" style="border-style:solid; border-color:black; border-width:1px;"
Hydrolase: domain 1 of 1, from 18 to 224: score 96.2, E = 1e-25
                  *->ikavvFDkDGTLtdgkeppiaeaiveaaaelgl.........lplee
                      ++av+FD+D+TL+d+ + + ++ + e+ ++l  + + +++ ++  +
      query    18    VRAVFFDLDNTLIDT-AGASRRGMLEVIKLLQSkyhykeeaeIICDK 63 
                  vekllgrgl.g.erilleggltaell...................d.evl
                  v  l +++ ++    ++  t ++ +  +++++ ++++  ++    ++
      query    64 VQVKLSKECfHpYSTCITDVRTSHWEeaiqetkggadnrklaeecYfLWK 113
                  glial.dklypgarealkaLkrrGikvailTggdr.naeallealgla.l
                    ++ ++  l +++++ l +L++  +++ +lT+gdr++++++ ea+++ ++
      query  114 STRLQhMILADDVKAMLTELRKE-VRLLLLTNGDRqTQREKIEACACQsY 162
                  fdviidsdevggvgpivvgKPkpeifllalerlgvkpeevgpevlmVGDg
                  fd+i++++e +        KP+p if + ++ lgv+p ++    +mVGD+
      query  163 FDAIVIGGEQK------EEKPAPSIFYHCCDLLGVQPGDC----VMVGDT 202
                  vnDapalaa.AGv.gvamgngg<-*
                  + +++ +  +AG+++++++n + 
      query  203 LETDIQGGLnAGLkATVWINKS    224 
|}
The alignments of the top-scoring domains of 2gfh (query) protein using Pfam 21.0 (Janelia Farm). ([http://pfam.janelia.org/ http://pfam.janelia.org])
* Shows the highest scored match --> hydrolase
* Score  = 96.2
* E value = 1e-25
----
, illustrate that 2gfh is a hydrolase. Profunc searches (Figure 17) on 2gfh also
show that it possesses hydrolase activity. The highest score for Gene Ontology (Figure 17) states it used for metabolism and possesses
phosphoglycolate phosphatase activity. Hydrolyase is an enzyme which catalyzes hydrolysis reaction (Figure 18), which is the addition of the
hydrogen and hydroxyl ions of water to a molecule with its consequent splitting into two or more simpler molecules. Hydrolase is the systematic
name for any enzyme of EC class 3.
'''Figure 18. '''Hydrolyase catalyze the hydrosis of the chemical bond between A and B, resulting of 2 simple molecules.
----
[[Image:Document9_01.png]]
The MSA for the query sequence and the other 35 sequences shows several conserved motifs. The 1<sup>st</sup> conserved motif
consists of almost invariant region of aspartic acid (D), only the 33<sup>rd</sup> protein (gi: <nowiki>|</nowiki>45552117<nowiki>|</nowiki>)
showing gap. The 2<sup>nd</sup> motif shows conserved and invariant of leucine (L), threonine (T), asparagine (N) and glycine (G). The
3<sup>rd</sup> motif shows 2 invariant amino acid residues of lysine (K), proline (P), valine (V), glycine (G), aspartic acid (D) and
isoleucine (I). This correlates with the study done by Maliekal ''et al'' and strongly suggested that the query protein is a phosphatase.
MSA of the query protein Neu5Ac phosphatase with 35 others proteins. Only the 60<sup>th</sup> – 70<sup>th</sup> and the
210<sup>th</sup> -300<sup>th</sup> amino acid sequence were shown to illustrate the conserved and invariant regions. The 3 boxed-up sequences
were either conserved or invariant regions.
[[Image:Document17_01.png]]
[[Image:Document17_03.png]]
'''Figure 17. (A)''' List of all matched protein name terms for 2gfh.''' (B) '''List of all matched Gene Ontology terms for 2gfh. The score in
red is a measure of how strongly the term is predicted from the hits obtained by the different methods. The scores in blue show each
method<nowiki>’</nowiki>s contribution to the total score (with the number of relevant sequences/structures shown in brackets in grey).
(http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/)
''E. coli ''HADs hydrolyze a wide range of phosphorylated metabolites, including carbohydrates, nucleotides, organic acids, and coenzymes.
Studies have shown that the most common substrates in metabolism such as glycolysis and pentose phosphate pathway (Figure 18). These enzymes
were fructose-1-phosphate, glucose-6-phosphate, mannose-6-phosphate, 2-deoxyglucose-6-phosphate, fructose-6- phosphate, ribose-5-phosphate, and
erythrose- 4-phosphate <sup>13</sup>.
[[Image:Document20_01.png]]
[[Image:Document20_02.png]]
'''Figure 20. '''The schematic diagrams of glycolysis and pentose phosphate metabolic pathways. The green arrows show the substrates that are hydrolyzed by HADs '''(A)''' Glycolysis pathway with substrates that are hydrolyze by HADs: glucose 6-phosphate, fructose 6-phosphate and dihydroxyacetone phosphate. '''(B)''' Pentose phostphate pathway with substrates that are hydrolyze by HADs: glucose-6-phosphate, fructose-6-phosphate, dihydroxyacetone phosphate, glyceraldehyde-3-phosphate, gluconate 6-phosphate and erythrose-4-phosphate.
(http://www.steve.gb.com/science/core_metabolism.html)
=='''Proposed Functions'''==
* Hydrolase Activity
* Magnesium Ion Binding
* N-acylneuraminate-9-phosphatase Activity
* Phosphoglycolate Phosphatase Activity
[http://amigo.geneontology.org/cgi-bin/amigo/go.cgi?view=details&depth=1&query=0016787 1. Hydrolase Activity]
[http://amigo.geneontology.org/cgi-bin/amigo/go.cgi?view=details&depth=1&query=0000287 2. Magnesium Ion Binding]
[http://amigo.geneontology.org/cgi-bin/amigo/go.cgi?view=details&depth=1&query=0050124 3. N-acylneuraminate-9-phosphatase Activity]
[http://amigo.geneontology.org/cgi-bin/amigo/go.cgi?view=details&depth=1&query=0008967 4. Phosphoglycolate Phosphatase Activity]
{| border="1" cellpadding="15" cellspacing="0"
|+'''Table of functions'''
|Catalytic activity
|N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate
|-
|Cofactor
|Magnesium (By similarity)
|-
|Enzyme regulation
|Inhibited by vanadate and calcium (By similarity)
|-
|Pathway
|Carbohydrate metabolism; aminosugar metabolism
|-
|Similarity
|Belongs to the haloacid dehalogenase-like hydrolase superfamily. NANP family
|}
Using ProFunc: Ligand-binding template search results for 2gfh.
Structural similarity: 91.5%
E-value < 1.00E-06 ( 7.22E-07)
Similarity score: 364.02
PBD id: 2hi0
Name: Hydrolase
Title: Crystal structure of putative phosphoglycolate phosphatase (yp_619066.1) from lactobacillus delbrueckii subsp. Bulgaricus atcc baa-365 at 1.51 a resolution
Source: Lactobacillus delbrueckii. Bacteria. Gene: yp_619066.1. Expressed in: escherichia coli.
Reaction: 2-phosphoglycolate + H2O = glycolate + phosphate
[[Image:Tree.JPG|thumb|Description|left]]
=='''GO Terms'''==
Polymer: haloacid dehalogenase-like hydrolase domain containing 4
Molecular Function: None
Biological Process: None
Cellular Component: None
=='''Function in Human'''==
haloacid dehalogenase-like hydrolase domain
(Found in OMIM)
Neu5Ac-9-PHOSPHATE PHOSPHATASE HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN-4; HDHD4
Gene map locus 20p11
Neu5Ac-9-phosphate phosphatase (Neu5Ac-9-Pase; EC 3.1.3.29) dephosphorylates Neu5Ac-9-phosphate to form N-acetylneuraminate (Neu5Ac), the main form of sialic acid in vertebrates that has important roles in protein-protein and cell-cell recognition.
CLONING
Maliekal et al. (2006) purified Neu5Ac-9-Pase from rat liver and isolated the phosphatase activity. Using SDS-PAGE analysis and tandem mass spectrometry, they identified a haloacid dehalogenase-like hydrolase domain containing-4 (HDHD4) protein. Purified recombinant human HDHD4 catalyzed the dephosphorylation of Neu5Ac-9-phosphate with a catalytic efficiency more than 2 orders of magnitude higher than for any other substrate tested. The 248-amino acid human HDHD4 protein has 3 motifs found in phosphatases of the haloacid dehalogenase (HAD) family: the first with 2 extremely conserved aspartates, the second comprising a conserved serine or threonine, and the third comprising a conserved lysine and 2 conserved aspartates.
GENE STRUCTURE
Maliekal et al. (2006) determined that the human HDHD4 gene contains 2 exons.
GENE FUNCTION
Maliekal et al. (2006) determined that the phosphatase activity of human Neu5Ac-9-Pase protein was dependent on the presence of Mg(2+) and was inhibited by vanadate and Ca(2+), which is characteristic of members of the HAD family of phosphatases.
----
info
N-acetylneuraminic acid phosphatase


Homologous to mouse (Mus musculus)
== Function of sulfatases ==


Haloacid Dehalogenase Like Hydrolase Domain Containing 4
Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates.
Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.


Classified as Hydrolase 


[http://www.ebi.ac.uk/thornton-srv/databases/profunc/index.html Infomation on ProFunc] (useful 2gfhA)


[http://www.ebi.ac.uk/pdbsum/ Information on PDBsum] (2gfh)


From [http://www.ebi.ac.uk/cgi-bin/sumtab?tool=fasta&jobid=fasta-20070515-07270763 FASTA] 
most likely function is N-acetylneuraminic acid phosphatase.


The haloalkanoate dehalogenase superfamily (HADSF) is one of the largest and most ubiquitous enzyme families identified to date, with over 3,000 members in organisms ranging from bacteria to humans. Remarkable diversity of chemistry and function has emerged through evolution of the HAD catalytic scaffold. Despite the name, the dehalogenases, which catalyze carbon group transfer, represent a minute fraction of the family. All other known catalytic activities are directed at phosphoryl transfer. Numerous proteins from the HADSF are found in each organism (29 in E. coli and 58 in humans, for example) where they perform a diverse collection of novel physiological functions in primary and secondary metabolism, membrane transport, signal transduction, and nucleic acid repair. http://biophysics.bumc.bu.edu/faculty/allen/allenpage/had.htm
== Functional site==
MSA data revealed some conserved residues on the sequence. They were mapped on the three dimantional structure.


Haloacid dehalogenases (E.C.3.8.1.2) are members of the haloacid dehalogenase superfamily, which also contains ATPases, phosphatases and epoxide hydrolases. They catalyse the conversion of α-halo-carboxylic acids to the corresponding hydroxyalkanoic acid by nucleophilic attack on the α-carbon by a conserved aspartic acid residue to form an ester intermediate, which is then further hydrolysed by a water molecule. There are three subtypes of haloacid dehalogenase based on substrate specificity; those that can use both enantiomers as substrates, those that act only on the L enantiomer and those that act only on the D enantiomer.
[[Image:Zn_Cl_surface]]


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Latest revision as of 09:44, 20 May 2008

The predicted function based on the evolution and structure

Hydrolase


Document18 01.png

Hydrolyase catalyze the hydrosis of the chemical bond between A and B, resulting of 2 simple molecules


  • Hydrolase
    • Catalyze hydrolysis reaction
    • Addition of the hydrogen and hydroxyl ions of water
    • Splitting into 2 or more simpler molecules
    • EC class 3


Function of sulfatases

Sulfatases are enzymes,which hydrolyse sulfate ester bonds of substrates. Most of the family members has shown to contain a highly conserved cystine residue and a bivalent metal binding site.



Functional site

MSA data revealed some conserved residues on the sequence. They were mapped on the three dimantional structure.

File:Zn Cl surface


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