Function: Siowwei

Results on Function Analysis

Table 2.1 Complete annotation of NUBP2 retrieved from UnitProtKB/Swiss-Prot

Names and origin

Protein names	Nucleotide-binding protein 2Also known as:     NBP 2
Gene names	Name:  NUBP2
Organism	Homo sapiens (Human)

General annotation (Comments)

Function	May contribute to formation of bipolar spindles (By similarity).
Subunit structure	Binds NUBP1 and KIFC1 (By similarity).
Subcellular location	Nucleus (By similarity). Note=Associated with nucleus during interphase and nucleocytoplasm surrounding mitotic spindles during early mitosis. Enriched around centrosomes during late mitosis (By similarity).
Tissue specificity	Widely expressed with highest expression in skeletal muscle.
Developmental stage	Expressed in fetal brain, lung, liver and kidney.
Sequence similarities	Belongs to the Mrp/NBP35 ATP-binding proteins family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-bindingNucleotide-binding
Gene Ontology (GO)
Molecular function	Nucleotide binding Traceable author statement. Source: ProtInc

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 271	271	Nucleotide-binding protein 2
Regions
	Nucleotide binding	22 – 29	8	ATP Potential

Table 2.2 Aligned sequence of 2ph1 protein

Aligned Sequences:

%-tage

Seq. id. identity Name

-------- -------- ----

Query - - Query sequence

O30288 100.0 - O30288 Nucleotide-binding protein.

O28015 79.8 - O28015 Nucleotide-binding protein.

B1I1P7 54.1 - B1I1P7 Putative uncharacterized protein precursor.

A5N3E5 50.7 - A5N3E5 Predicted nucleotide-binding protein.

B0ACV7 55.7 - B0ACV7 Putative uncharacterized protein.

A8SJJ8 43.5 - A8SJJ8 Putative uncharacterized protein.

O27244 49.6 - O27244 Nucleotide-binding protein (Putative ATPase).

Q3AQ21 48.3 - Q3AQ21 ATP-binding protein, Mrp/Nbp35 family.

Q48LT6 45.3 - Q48LT6 ATP-binding protein, Mrp/Nbp35 family.

A1RYM9 46.4 - A1RYM9 MRP protein-like protein.

Table 2.3 The diagram and table below show the 3 sequence motifs found in the sequence

Table 2.4 The diagram and table below show the 1 sequence of superfamily found in the sequence

Figure 1. Protein-protein interactions search using 2ph1 sequence

[[Image:]]

Your Input: [[Image:]] AF2382 Nucleotide-binding protein (254 aa) (Archaeoglobus fulgidus) Predicted Functional Partners:			[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]
[[Image:]]	AF2381	Iron-sulfur cluster binding protein (283 aa)	•		•						0.927
[[Image:]]	AF2380	Iron-sulfur cluster binding protein (262 aa)	•								0.894
[[Image:]]	metG	Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS) (658 aa)	•						•		0.827
[[Image:]]	AF2378	Hypothetical protein {UniProtKB/TrEMBL-O30292} (144 aa)	•								0.808
[[Image:]]	AF_2379	Hypothetical protein AF_2379 (85 aa)	•								0.808
[[Image:]]	AF2383	Hypothetical protein {UniProtKB/TrEMBL-O30287} (217 aa)	•								0.729
[[Image:]]	AF2377	Hypothetical protein {UniProtKB/TrEMBL-O30293} (107 aa)	•								0.704
[[Image:]]	AF1185	Iron-sulfur cluster binding protein (131 aa)			•						0.485
[[Image:]]	suhB	Inositol-1-monophosphatase (EC 3.1.3.25) (IMPase) (Inositol-1- phosphatase) (I-1-Pase) (252 aa)	•		•						0.472
[[Image:]]	AF2385	Molybdopterin oxidoreductase

Figure 2. Protein-protein interactions search using NUBP2 sequence

[[Image:]]

Your Input: [[Image:]] NUBP2 Nucleotide binding protein 2 (NBP 2) (271 aa) (Homo sapiens) Predicted Functional Partners:			[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]
[[Image:]]	IGFALS	Insulin-like growth factor-binding protein complex acid labile chain precursor (ALS) (605 aa)							•		0.675
[[Image:]]	MAPK8IP3	C-jun-amino-terminal kinase interacting protein 3 (JNK-interacting protein 3) (JIP-3) (JNK MAP kinase scaffold protein 3) (Mitogen- activated protein kinase 8-interacting protein 3) (1342 aa)							•		0.673
[[Image:]]	REG3G	Regenerating islet-derived protein 3 gamma precursor (Reg III-gamma) (Pancreatitis-associated protein 1B) (PAP IB) (175 aa)							•		0.619
[[Image:]]	ENSP00000262027	Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS) (900 aa)	•						•		0.594
[[Image:]]	RANBP1	Ran-specific GTPase-activating protein (Ran binding protein 1) (RanBP1) (201 aa)							•		0.525
[[Image:]]	KIF11	Kinesin-like protein KIF11 (Kinesin-related motor protein Eg5) (Kinesin-like spindle protein HKSP) (Thyroid receptor interacting protein 5) (TRIP5) (Kinesin-like protein 1) (1056 aa)							•		0.514
[[Image:]]	SEPP1	Selenoprotein P precursor (SeP) (381 aa)							•		0.491
[[Image:]]	NR3C2	Mineralocorticoid receptor (MR) (988 aa)							•		0.477
[[Image:]]	CYPA	Peptidyl-prolyl cis-trans isomerase A (EC 5.2.1.8) (PPIase) (Rotamase) (Cyclophilin A) (Cyclosporin A-binding protein) (165 aa)							•		0.443
[[Image:]]	WDR39	WD-repeat protein 39 (Ciao 1 protein) (339 aa)		•					•		0.414

Figure 3. NUBP2 expressions in human and mouse tissues

[[Image:]]