Function for haloacid dehalogenase-like hydrolase domain containing 2

Haloacid dehalogenase-like hydrolase domain containing 2 (2HO4:A,B)

Where's does this name come from?

Hydolase indicates it's main molecular function, hydrolase activity. /Link

Definition of hydrolase activity:

Catalysis of the hydrolysis of various bonds

e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.

Hydrolase is the systematic name for any enzyme of EC class 3.

---

The name Domain containing 2 illustrate it has 2 domains

Sequence and Secondary Structure /Link

From the above diagram, we see 2 domains color in blue and purple showed separately.

Type: polypeptide(L)

Length: 259 residues

Secondary Structure: 44% helical (14 helices; 123 residues) 17% beta sheet (12 strands; 48 residues)

2HO4Aa: pdp domain 2HO4Aa (in purple)

2HO4Ab: pdp domain 2HO4Ab (in blue)

But this does not conclude all it's function

---

Protein Data Bank (PDB)

Ligands and Prosthetic Groups

ID	Name Chemical	Formula	Weight Ligand	Link
PO4	Phosphate Ion	O4 P 3-	94.971	/View
MG	Magnesium Ion	Mg 2+	24.305	/View
MSE	Selenomethionine	C5 H11 N O2 Se	196.107	/View

/PBD Biology and Chemistry Report

---

Mouse Genome Informatics(MGI)

/Gene Ontology Classifications

We found 5 Category of Function or Process with evidence.

GO Annotations in Tabular Form


Category

Classification

Term Evidence

Inferred From

Ref(s)


Molecular

Function catalytic

activity

IEA

InterPro:IPR005834

J:72247


Molecular Function

hydrolase activity

IEA

InterPro:IPR006357

J:72247


Molecular Function

hydrolase activity

IEA

SP_KW:KW-0378

J:60000


Molecular Function

magnesium ion binding

IEA

SP_KW:KW-0460

J:60000


Biological Process

metabolic process

IEA

InterPro:IPR005834,InterPro:IPR006357

J:72247



(pre)
Gene Ontology Evidence Code Abbreviations:

  IC Inferred by curator 
  IDA Inferred from direct assay 
  IEA Inferred from electronic annotation 
  IGI Inferred from genetic interaction 
  IMP Inferred from mutant phenotype 
  IPI Inferred from physical interaction 
  ISS Inferred from sequence or structural similarity 
  NAS Non-traceable author statement 
  ND No biological data available 
  RCA Reviewed computational analysis 
  TAS Traceable author statement 
(/pre)






So where's the evidence from?
The evidence was done by Mammalian Orthology Load, which information was derived from Homologene National Center for Biotechnology Information.  This polymer was reviewed in 2004 and record with J Number: J:90500.

The Mammalian Orthology Query Results shows the Orthology Evidence were used both Amino acid sequence comparison 
and NT Nucleotide sequence comparison.  With species of human, mouse(laboratory), rat, chimpanzee and dog(domestic) all contain this polymer.

/more information



What about it's functional partners
From /STRING uses orthology information from the excellent COG database, we predicted it's functional partners.

From the result of occurrence we discover KIAA0423 annotation, which is still not available (1723 aa) is it best suit predicted Functional Partners.  / more information



The above result shows using protein sequence of ploymer to compare in difference species and their sequence conservation.

e.g. Pan troglodytes and Homo sapiens are two species with this polymer similiar



How does hydrolase activity?
The HAD (haloacid dehalogenase) superfamily of hydrolases contains enzymes such as L-2-haloacid dehalogenase, epoxide hydrolase and a variety of phosphatases








HDHD2 (259 aa) (Homo sapiens) is highly conservatity to Pan troglodytes, Macaca mulatta, Rattus norvegicus, Mus musculus species.

Predicted Functional Partners:  KIAA0423 annotation not available (1723 aa)

/ ProKnow

/ Gene Ontology Classifications 

/ PDB sum

/ Mammalian Orthology Proteins

http://symatlas.gnf.org/SymAtlas/symquery?q=212508





  
PO4
 
  
MG
 
  
MSE