Functional Analysis Of 1zkd

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Profunc Analysis

Best scores for Methyltransferase: in SSM (Secondary Structure Matching), LIG(Ligand Template Matches) and REV (Reverse Template Matches)

http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/profunc/GetResults.pl?source=profunc&user_id=1zkd&code=none

Superfamily search against HMM library found motif similar to S-adenosyl-L-methionine-dependent methyltransferases (1g8a) which is a RNA binding protein:

http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1g8a

http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?sunid=53335

No DNA-binding motifs (Helix-turn-helix) were found in the ProFunc search.



Search against NCBI Protein Database for "S-adenosyl-L-methionine-dependent Methyltransferase" limited to RefSeq Sequences

101 hits --> saved all as FASTA file "search150507.fasta"

deleted sequences of excessive length from fasta file:

gi15234111 gi88860170 gi77360608 gi119501377 gi126138018

added sequence of 1zkd to fasta file --> saved file as "search150507_1.fasta"