Functional analysis of 2qgn: Difference between revisions

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[[Image:protein interaction.jpg]]
[[Image:protein interaction.jpg]]


* mutL DNA mismatch repair protein mutL (637 aa)
* Input protein - miaA :


* hfq Protein hfq {UniProtKB/Swiss-Prot-Q9KAC4} (78 aa)  
* mutL : DNA mismatch repair protein mutL (637 aa)  


* mutS DNA mismatch repair protein mutS (865 aa)  
* hfq : Protein hfq {UniProtKB/Swiss-Prot-Q9KAC4} (78 aa)  


* BH2367 BH2367 protein {UniProtKB/TrEMBL-Q9KAC2} (256 aa)  
* mutS : DNA mismatch repair protein mutS (865 aa)  


* BH2362 BH2362 protein {UniProtKB/TrEMBL-Q9KAC7} (418 aa)  
* BH2367 : BH2367 protein {UniProtKB/TrEMBL-Q9KAC2} (256 aa)  


* BH3154 Protease specific for phage lambda cII repressor (310 aa)  
* BH2362:BH2362 protein {UniProtKB/TrEMBL-Q9KAC7} (418 aa)  


* BH3155 Protease specific for phage lambda cII repressor (319 aa)  
* BH3154 : Protease specific for phage lambda cII repressor (310 aa)  


* BH0545 BH0545 protein {UniProtKB/TrEMBL-Q9KFD6} (157 aa)  
* BH3155: Protease specific for phage lambda cII repressor (319 aa)
 
* BH0545 :BH0545 protein {UniProtKB/TrEMBL-Q9KFD6} (157 aa)  
   
   
* BH1020 BH1020 protein {UniProtKB/TrEMBL-Q9KE38} (391 aa)  
* BH1020 : BH1020 protein {UniProtKB/TrEMBL-Q9KE38} (391 aa)  


* dapF Diaminopimelate epimerase (EC 5.1.1.7) (DAP epimerase) (286 aa)
* dapF : Diaminopimelate epimerase (EC 5.1.1.7) (DAP epimerase) (286 aa)

Revision as of 09:15, 3 June 2008

Molecular function and biological processes of this enzyme was obtained from the ProKnow server. Molecular function of this enzyme is ATP binding. This means that the enzyme interacts selectively with ATP at the 5'end and acts as a universally important coenzyme and enzyme regulator. On the other hand, the biological process of this enzyme is tRNA modification. This involves the covalent alteration of one or more nucleotides within the tRNA, thus leading to a change in the properties of the tRNA.

Molecular Function


PROKNOW2- molecular function.png


Biological Process


PROKNOW-biological process.png


Localisation of Expression

Mouse gene atlas.png


Human geneAtlas.png


using the motif search, The C2-H2 Zinc finger motif found in tRNA-IPT also contributes to the enzyme's function. This motif is commonly found in nucleic acid-binding proteins and is composed of 25 to 30 amino acid residues.

Motif ZINC_FINGER_C2H2_1 in your sequence

Prosite ID: ZINC_FINGER_C2H2_1 (PS00028)

Description: Zinc finger C2H2 type domain signature.

Pattern: C-x(2,4)-C-x(3)-[LIVMFYWC]-x(8)-H-x(3,5)-H.

Appearance: Position Found Motif 396..418 CDLCDRIIIGDREWAAHIKSKSH

Sequence: MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVE GLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGG TNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHD KRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLD KRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLE TSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLE PALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLN QLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV



A schematic representation of a zinc finger domain is shown below:

                                x  x
                              x      x
                             x        x
                             x        x
                             x        x
                             x        x
                              C      H
                            x   \  /   x
                           x     Zn     x
                            x  /    \  x
                              C      H
                     x x x x x        x x x x x


http://ca.expasy.org/cgi-bin/nicedoc.pl?PDOC00028

GENOMIC CONTEXT In bacteria, the gene encoding this enzyme is known as miaA while the human and S. cerevisaea is TRIT1 and mod5 respectively. Although gene name differ in different organisms, they all synthesize enzyme of the same function.

Sequence Motif

Motif.jpg

Functional partners of query protein

Protein interaction.jpg

  • Input protein - miaA :
  • mutL : DNA mismatch repair protein mutL (637 aa)
  • hfq : Protein hfq {UniProtKB/Swiss-Prot-Q9KAC4} (78 aa)
  • mutS : DNA mismatch repair protein mutS (865 aa)
  • BH2367 : BH2367 protein {UniProtKB/TrEMBL-Q9KAC2} (256 aa)
  • BH2362:BH2362 protein {UniProtKB/TrEMBL-Q9KAC7} (418 aa)
  • BH3154 : Protease specific for phage lambda cII repressor (310 aa)
  • BH3155: Protease specific for phage lambda cII repressor (319 aa)
  • BH0545 :BH0545 protein {UniProtKB/TrEMBL-Q9KFD6} (157 aa)
  • BH1020 : BH1020 protein {UniProtKB/TrEMBL-Q9KE38} (391 aa)
  • dapF : Diaminopimelate epimerase (EC 5.1.1.7) (DAP epimerase) (286 aa)