Hypothetical protein Results: Difference between revisions

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'''Figure 4:''' Curved phylogenetic tree with bootstrap values, obtained using MEGA 4.0 [2]
'''Figure 4:''' Curved phylogenetic tree with bootstrap values, obtained using MEGA 4.0 [2]
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'''Radiation tree:'''
'''Radiation tree featuring selected bootstrap values:'''
[[Image:radiationtreexx22.jpg]]
[[Image:radiationtreexx22.jpg]]
'''Figure 5:''' Phylogenetic tree representing radiation of Ribose/Fucose transporter, Obtained using MEGA 4.0 [2] and manually edited in Microsoft Power Point 2007.
'''Figure 5:''' Phylogenetic tree representing radiation of Ribose/Fucose transporter, Obtained using MEGA 4.0 [2] and manually edited in Microsoft Power Point 2007.



Revision as of 04:43, 15 June 2009

Abstract | Introduction | Method | Results | Discussion | Conclusion | References

Evolution

Sequences:

Sequences (FASTA format) gathered for MSA (including query), available via the following link: [1]


Multiple Sequence Alignment: Hypotheticalmsa1xx.jpg Figure 3: Multiple sequence alignment generated using Clustalx 1.83 [1].


Original Curved Bootstrapped Tree: Hypotreexx21.jpg Figure 4: Curved phylogenetic tree with bootstrap values, obtained using MEGA 4.0 [2]


Radiation tree featuring selected bootstrap values: Radiationtreexx22.jpg

Figure 5: Phylogenetic tree representing radiation of Ribose/Fucose transporter, Obtained using MEGA 4.0 [2] and manually edited in Microsoft Power Point 2007.

Structure

Function

Blasthp.jpg

The results from the blast search showed a conserved FucU/RbsD domain.


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Interpro Scan result

Interpro1.gif

This image shows the results of a InterProScan. The InterProScan search identifies sequence motifs from several databases.These results show that the target protein belongs to the RbsD/FucU superfamily (Purple and red lines) The gray line shows an possible binding histadine site.


PDB Sequence Match

PDB1.jpg

This PDB scan compares structure alignment. The highest structural match with our sequence is shown to be involved in an d-ribose high affinity transport system. This supports the theory that our hypothetical protein is involved in the ABC transport system of ribose in prokaryote cells.