Difference between revisions of "Hypothetical protein Results"

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=='''Function'''==
 
=='''Function'''==
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===Blast Search===
  
 
[[Image:blasthp.jpg]]
 
[[Image:blasthp.jpg]]
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The results from the blast search showed a conserved FucU/RbsD domain.
 
The results from the blast search showed a conserved FucU/RbsD domain.
  
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===String Analysis===
  
[[hypothetical protein LOC282969 isoform 1 |  Go Back To Main Page]]
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[[Image:String.jpg]]
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This string analysis shows possible protein - protein interactions. The red protein in the middle represents the target protein and the three surrounding proteins represent interacting proteins.
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The larger yellow and green proteins may be releated due to cooccurrence, where as the smaller blue protien may be related due to repeatedly occurring in close proximity to the target protein, however the posible interactions shown in the analysis are inconclusive and unsubstantiated. experimental evidence is needed to prove such relationships<br>
  
  
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This PDB scan compares structure alignment. The highest structural match with our sequence is shown to be involved in an d-ribose high affinity transport system. This supports the theory that our hypothetical protein is involved in the ABC transport system of ribose in prokaryote cells.
 
This PDB scan compares structure alignment. The highest structural match with our sequence is shown to be involved in an d-ribose high affinity transport system. This supports the theory that our hypothetical protein is involved in the ABC transport system of ribose in prokaryote cells.
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[[hypothetical protein LOC282969 isoform 1 |  Go Back To Main Page]]

Revision as of 04:43, 15 June 2009

Abstract | Introduction | Method | Results | Discussion | Conclusion | References

Evolution

Sequences:

Sequences (FASTA format) gathered for MSA (including query), available via the following link: [1]


Multiple Sequence Alignment: Hypotheticalmsa1xx.jpg Figure 3: Multiple sequence alignment generated using Clustalx 1.83 [1].


Original Curved Bootstrapped Tree: Hypotreexx21.jpg Figure 4: Curved phylogenetic tree with bootstrap values, obtained using MEGA 4.0 [2]


Radiation tree featuring selected bootstrap values: Radiationtreexx22.jpg

Figure 5: Phylogenetic tree representing radiation of Ribose/Fucose transporter, Obtained using MEGA 4.0 [2] and manually edited in Microsoft Power Point 2007.

Structure

Function

Blast Search

Blasthp.jpg

The results from the blast search showed a conserved FucU/RbsD domain.

String Analysis

String.jpg

This string analysis shows possible protein - protein interactions. The red protein in the middle represents the target protein and the three surrounding proteins represent interacting proteins. The larger yellow and green proteins may be releated due to cooccurrence, where as the smaller blue protien may be related due to repeatedly occurring in close proximity to the target protein, however the posible interactions shown in the analysis are inconclusive and unsubstantiated. experimental evidence is needed to prove such relationships


Interpro Scan result

Interpro1.gif

This image shows the results of a InterProScan. The InterProScan search identifies sequence motifs from several databases.These results show that the target protein belongs to the RbsD/FucU superfamily (Purple and red lines) The gray line shows an possible binding histadine site.


PDB Sequence Match

PDB1.jpg

This PDB scan compares structure alignment. The highest structural match with our sequence is shown to be involved in an d-ribose high affinity transport system. This supports the theory that our hypothetical protein is involved in the ABC transport system of ribose in prokaryote cells.


Go Back To Main Page