Materials and Methods

Query Sequences (2gfh)

See Evolution

Multiple Sequence Alignments (msa)

Phylip programme was used for the purpose of obtaining a phylogentic tree to determine the relationship of the proteins from individual organisms.

The various programmes used were again obtained from the DVD.

Prodist (within Phylip) was used to calculate the distance matrix. The calculation method selected was as using PAM-Dayhoff.

Neighbor (also found within Phylip) was next used to form the phylogenetic tree, using the distance matrix calculation obtained. The “Input order of species” option was set to “Random” when generating the tree, with a random odd number also given.

Treeview programme was used to view the final tree.

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