Methods 2ece: Difference between revisions

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== STRUCTURAL ANALYSIS ==
Methods employed to obtain the analytical data was devided into three categories


[[Methods for evolutionary analysis]]


[[Methods for structural analysis]]


[[Image:2ece_bio_r_500structure.jpg]]
[[Methods for functional analysis]]


X-ray structure of hypothetical selenium-binding protein from Sulfolobus tokodaii, ST0059 ( http://www.proteopedia.org/wiki/index.php/2ece ) and the JenaLib Jmol viewer showing SBP 1 secondary structure [http://www.imb-jena.de/cgi-bin/3d_mapping.pl?CODE=2ece&MODE=asymmetric]
Back to [[Selenium binding protein]]
 
 
 
[[Image:movie0001.png]]
 
 
[[Image:secondary structure.gif]]
 
 
[[Image:pdb_cartoon_2ece.png]]
 
STRUCTURAL COMPARISONS
 
[[Image:surface of 2ece.png]]
 
Surface Structure of SBP: Note the few clefts compared to the DNA isomerase shown below.
 
 
[[Image:1YUA SURFACE STRUCTURE.JPG]]]]]
 
[http://www.ncbi.nlm.nih.gov/entrez/query/static/gifs/entrez_struc.gif]
 
[[Image:Rat fatty acid binding protein 2IFB.jpg]]
 
Rat fatty acid binding protein 2IFB, a was found to have 92.5% homology to 14 KDa Selenium binding protein purified from rat liver using column chromatography and SDS-Gel techniques.([[ref:3]])
 
 
 
 
Explore SBP features and structural summary here [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=61601].The domains of SBP are shown here [http://www.ncbi.nlm.nih.gov/sites/entrez?db=domains&cmd=search&term=2ece]
Notice how the domains are similar to the putative Isomerase domains of E.coli below.
 
 
'''1RI6 DOMAINS'''                                 
 
 
[[Image:1RI6 A 0.png]]                             
                                                   
 
[[Image:1RI6 A 1.png]]
 
                                                     
[[Image:1RI6 A 2.png]]
 
 
[[Image:1RI6 A 3.png]]
 
 
                                                     
'''2ECE DOMAINS''' 
 
 
[[Image:2ece A 3.png]]
 
 
 
[[Image:2ece A 2.png]]
 
 
[[Image:2ece A 1.png]]
 
 
[[Image:2ece A 0.png]]
 
 
 
 
 
 
 
 
 
 
 
                       
 
 
 
[[Image:Ligand of bovine.png]]
 
 
(Complex Of Bovine Odorant Binding Protein (Obp) With A Selenium Containing Odorant)"Image:Ligand of bovine.png" [[http://compbio.chemistry.uq.edu.au/mediawiki/upload/2/23/Ligand_of_bovine.png]]
 
== SEQUENCE ANALYSIS ==
Selenium binding protein 1 (SELENBP1)
SELECTED PROTEIN SIMILARITIES
Comparison of sequences in UniGene with selected protein reference sequences. The alignments can suggest function of a gene.
[http://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=632460]

Latest revision as of 15:01, 9 June 2008

Methods employed to obtain the analytical data was devided into three categories

Methods for evolutionary analysis

Methods for structural analysis

Methods for functional analysis

Back to Selenium binding protein