Methods and Materials: Difference between revisions
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'''Following the evolution of the protein''' | '''Following the evolution of the protein''' | ||
The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. | The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. The program Phylip was used. The calculations from the matrix distance calculation were transformed by using the subprogram in Phylip, Neighbor. | ||
Revision as of 13:44, 4 June 2007
Following the evolution of the protein
The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. The program Phylip was used. The calculations from the matrix distance calculation were transformed by using the subprogram in Phylip, Neighbor.
