Methods and Materials: Difference between revisions
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'''Following the evolution of the protein''' | '''Following the evolution of the protein''' | ||
The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. The program Phylip was used. The calculations from the matrix distance calculation were transformed by using the subprogram in Phylip, Neighbor. Afterwards, the bootstrap values | The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. The program Phylip was used. The calculations from the matrix distance calculation were transformed by using the subprogram in Phylip, Neighbor. Afterwards, the bootstrap values for the sequences was calculated using Phylip. THe resulting tree with the bootstrap values displayed was opened by using the program Treefile. | ||
Revision as of 13:54, 4 June 2007
Following the evolution of the protein
The protein sequence was blasted in the NCBI website. The blasted sequence was scanned using a non-redundand database to obtain the most significant sequences. 57 sequences with significant E-values were recovered. 55 of these sequences were used to perform a multiple alignment sequence by using the program ClustalX. When this was completed, a distance matrix calculation was performed on the sequences. The program Phylip was used. The calculations from the matrix distance calculation were transformed by using the subprogram in Phylip, Neighbor. Afterwards, the bootstrap values for the sequences was calculated using Phylip. THe resulting tree with the bootstrap values displayed was opened by using the program Treefile.
