Methods for structural analysis

Literature was searched for Selenium Binding Protein in Pubmed, Google scholar and other databases. The SBP amino acid sequence in FASTA format was retrieved from the protein database. The structural analysis was done using Dali to compare SBP with other protein with knonwn functions. Furthermore, Profunc and InterPro were as well searched for data about SBP. Further searches were done for other metal binding pproteins for clues of how Selenium binds to the apoprotein without the selenocysteine formation.

Results from Dali were received and the structures with high identity were compared using pymol and in literature.

SABLE protein structure prediction server was used to determine the type of the protein SBP is.