Mz
1. Psi-BLAST search and sequences selection:
- Selected organisms (33) across the three domains include:
Archae (6) - A3 (Methanococcus voltae A3), 6A8 (Candidatus Methanoregula boonei 6A8), C.symbiosum (Cenarchaeum symbiosum A), GSS1 (Thermoplasma volcanium GSS1), A. fulgidus (Archaeoglobus fulgidus DSM 4304), uncultured (uncultured methanogenic archaeon RC-I)
Bacteria (7) - Goettinger (Syntrophomonas wolfei subsp. wolfei str. Goettingen), Lettingae (Thermotoga lettingae), ATCC 824 (Clostridium acetobutylicum ATCC 824), MIT 9211 (Prochlorococcus marinus str. MIT 9211), BAV1 (Dehalococcoides sp. BAV1), DSM 4136 (Verrucomicrobium spinosum DSM 4136), DSM 245 (Chlorobium limicola DSM 245)
Eukaryota (20)- Red Rice Japanese / Indian (Oryza sativa), Neurospora (Neurospora crassa OR74A), C.reinhardtii (Chlamydomonas reinhardtii), C.Elegans (Caenorhabditis elegans), Thale cress (Arabidopsis thaliana), Fission yeast (Schizosaccharomyces pombe 972h-), Brewer’s yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio), Drosophila.pseudo (Drosophila pseudoobscura), Fruit Fly (Drosophila melanogaster), western clawed frog (Xenopus tropicalis), African clawed frog (Xenopus laevis), Chicken (gallus gallus), Mouse (mus mucus), Rat (Rattus norvegicus), Cow (Bos taurus), Chimpanzee (Pan troglodytes), Human (homo sapiens).
2. ClustalX - multiple sequence alignment and conserved motifs:
Figure 1: P-loop folding relies on at least two motifs, S-G-K-G(2)-V-G-K-[ST] and D-X-D-[VFLIM]-X-G.
Figure 2: Another two conserved alpha and beta NUBP/MRP, which are likely to associate with the folding of p-loop components.
3. Phylogenic Trees and evolution:
Figure 3: Nucleotide binding protein 1 (long form) might appear earlier than nucleotide binding protein 2 (short form) during evolution.
