Protein Evolution

From MDWiki
Revision as of 05:54, 15 May 2007 by S4097429 (talk | contribs)
Jump to navigationJump to search

Conservation of MIF4GD in Eutheria (Placental Mammals)

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=homologene&dopt=HomoloGene&list_uids=41389


Conservation of the protein structure in many organisms, including Pan troglodytes (chimpanzee), Mus musculus (house mouse), Rattus norvegicus (brown rat), Bos taurus (domestic cow) and Danio rerio (zebrafish).


SUMMARY

The protein sequence was transformed into FASTA format

File:FASTA2.txt

Using Command Prompt and the CD, BLASTed the protein sequence. First used a vertebrate_other database (which produced only 12 significant results), before was finally able to access the nonredundant database with 57 homologous matching sequences and several different species including Danio rerio, Homo sapiens, Mus musculus, Bos taurus, Xenopus tropicalis, Rattus norvegicus, Xenopus laevis, Canis familiaris, Pan troglodytes, Drosophila melangaster, Drosophila pseudoobscura, Aedes aegypti (mosquito) and Tetraodon nigroviridis (green spotted pufferfish).

The protein seems to be conserved throughout the animal kingdom. Must have a quite important function. Hopefully when the phylogenetic tree is constructed, will get more insight into the evolution of MIF46 domain containing protein.


File that shows the significant sequences obtained from the non-redundant database.

File:Sequences.txt


File that shows the id numbers of the significant sequences found from the non-redundant database.

[[]]