Protein Structure

The so-called MIF4G domain found in several other proteins involved in RNA metabolism. (Mazza et al.)(PDB no: 1H6K)

- Coordinates assembly of translational machinery

DALI results

1. 1. SUMMARY: PDB/chain identifiers and structural alignment statistics

 NR. STRID1 STRID2  Z   RMSD LALI LSEQ2 %IDE REVERS PERMUT NFRAG TOPO PROTEIN
  1: 3028-A 2i2o-A 37.1  0.0  206   206  100      0      0     1 S    STRUCTURAL GENOMICS, UNKNOWN FUNCTION 	eif4g-like prote
  2: 3028-A 1hu3-A 15.2  2.7  164   204   23      0      0    12 S     TRANSLATION 	eif4gii fragment (eukaryotic initiation f
  3: 3028-A 1uw4-B 10.9  3.6  164   247    9      0      0    12 S    NONSENSE MEDIATED MRNA DECAY PROTEIN 	regulator of nons
  4: 3028-A 1h6k-A 10.6  3.9  175   728   11      0      0    11 S     NUCLEAR PROTEIN 	cbp80 fragment (ncbp 80 kda subunit, 
  5: 3028-A 2db0-A  8.1  3.3  148   239   14      0      0    13 S    PROTEIN BINDING 	253aa long hypothetical protein (hypot
  6: 3028-A 1b3u-A  8.0 27.7  150   588   13      0      0    13 S    SCAFFOLD PROTEIN 	protein phosphatase pp2a fragment

ClustalW Results

Using the scores and results from DALI, a multiple sequence alignment was created with ClustalW. The sequences used were: (i)2i2o Structural Genomics, unknown function (ii)1hu3 Translation eif4gii fragment (iii)1uw4 nonsense mediated mRNA decay protein (iv)1h6k Nuclear Protein (refer to DALI table if unsure)

Generally, the alignment shows pathetic homology between these 4 sequences, which were already the best sequences that DALI could give. To View results, click here [1]

Articles

A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery. (PDB no:1HU3) [2]

Crystal structure of the human nuclear cap binding complex. (PDB no: 1H6K) [3]

Novel eIF4G domain homologues linking mRNA translation with nonsensemediated mRNA decay [4]

• This article is quite relevant to protein function