Pyridoxal Phosphatase Conclusion

In conclusion, the results suggested that the team is on the right track, in terms of characterizing the protein. In the evolutionary component of this paper, the conserved regions obtained from Multiple Sequence Alignment (MSA) was visualized using PyMOL. This gave the team a rough idea on the location of 2cfsA's catalytic site. Evolutionary relatedness was then characterised through the creation of a phylogenetic tree. During the structural component, based on the information obtained from Almo et al., 2007, and that of PDBsum - the LIGPLOTs, in particular - PyMOL was once again used to generate a three dimensional view of the protein's catalytic site. Similarities between both visualizations supported the theory that the deduced catalytic site could indeed be the actual one.