Results ERp18

Evoltuion of MIF4G Domain Containing Protein

Results notes

First identified in Dario rerio (zebra fish)

Eukaryotic gene - derived gene

Protein conserved throughout the Metazoans (animals)

Structure of MIF4G Domain Containing Protein

From the PDB search, the structure was revealed to be similar to “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein. The protein was isolated form Danio rerio and expressed in Escherichia coli. It is formed from two chains, with two chemical components, nickel (Ni2+) and selenomethionine (C5H11NO2Se) as additions to the protein. The NCBI Entrez search revealed the protein to be a domain of the eIF4G-like protein.

Structure of MIF4G (PDB: 1hu3)

Both Pfam and InterPro identified the protein as the Middle domain of eIF4G, termed MIF4G. MIF4G consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.