Pyridoxal Phosphatase function: Difference between revisions

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Vitamin B6, Pyridoxal Phosphatase,
                      ''Pyridoxal 5'-phosphate + H2O [[Image:Reaction Arrow.jpg]] pyridoxal + phosphate''
 


===General Annotation===
===General Annotation===
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===Known Functions===
===Known Functions===


'''Amino Acid Metabolism'''
- transamination


- transsulfuration


'''Amino Acid Metabolism'''
- selenoamino acid metabolism


2cfsA is involved in almost all amino acid metabolism. Some of these amino acid metabolism include transamination, transsulfuration, selenoamino acid metabolism and conversion of typtophan to niacin.
- conversion of typtophan to niacin.




'''Gluconeogenesis'''
'''Gluconeogenesis'''


2cfsA can catalyse transamination reactions that are essential for providing amino acids as a substrate for gluconeogenesis. 2cfsA is also required as a coenzyme for glycogen phosphorylase, which in turn catalyse glycogenolysis.
- Catalyse transamination reactions for gluconeogenesis.
 
- Coenzyme for glycogen phosphorylase, which catalyse glycogenolysis.




'''Neurotransmitter Synthesis'''
'''Neurotransmitter Synthesis'''


Pyridoxal phosphate-dependent enzymes catalyses the biosynthesis of serotonin, epinephrine, norepinephrine and gamma-aminobutyric acid(The four important neurotranmsitters).
- Coenzyme for Pyridoxal phosphate-dependent enzymes, which catalyses the biosynthesis of serotonin, epinephrine, norepinephrine and gamma-aminobutyric acid(The four important neurotranmsitters).




'''Histamine Synthesis'''
'''Histamine Synthesis'''


2cfsA is involved in the metabolism of histamine.
- Metabolism of histamine.




'''Hemoglobin Synthesis and Function'''
'''Hemoglobin Synthesis and Function'''


Pyridoxal phosphate aids in the synthesis of heme and can also bind to two sites on hemoglobin to enhance the oxygen binding of hemoglobin.
- Synthesis of heme  
 
- Binds to two sites on hemoglobin, enhancing the oxygen binding of hemoglobin.




'''Gene Expression'''
'''Gene Expression'''


Pyridoxal phosphate has been implicated in increasing or decreasing the expression of certain genes. Increased intracellular levels of 2cfsA will lead to a decrease in the transcription of glucocorticoid hormones. Also, vitamin B6 deficiency will lead to the increased expression of albumin mRNA. Also, pyridoxal phosphate will influence gene expression of glycoprotein IIb by interacting with various transcription factors. The result is inhibition of platelet aggregation.
- Increases or decreases the expression of certain genes.


===Sequence homology===
===Sequence homology===


[[Image:FASTA2.jpg|left|thumb|1000px|Fig 18. Results from FASTA showing five highly related enzymes to 2cfsA]]<BR>
[[Image:FASTA2.jpg|center|thumb|1000px|Fig 18. Results from FASTA showing five highly related enzymes to 2cfsA]]<BR>


===Structural Similarities===
===Structural Similarities===
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[[Image:String3.jpg|center|thumb|500px|Fig 22. Diagram on protein-protein interaction of 2cfsA.]]<BR>
[[Image:String3.jpg|center|thumb|500px|Fig 22. Diagram on protein-protein interaction of 2cfsA.]]<BR>


Homolog interactions suggests that 2cfsA maybe a cofactor of HSPD1 and play a role in protein folding. The search also revealed that TS11 is a neighbour gene for 2cfsA for 16 different genomes, hence it is possible that 2cfsA may actually bind to TS11 and function as a coenzyme for asparagine synthetase.
Functional Prediction:
 
Cofactor of HSPD1 and play a role in protein folding.  
 
Coenzyme for asparagine synthetase by interacting with TS11.




[[Pyridoxal Phosphatase| Back To Main Page]] | [[Pyridoxal Phosphatase structure| Back [Structure]]] | [[BIOL3004 2008| Back to BIOL3004 2008]]
[[Pyridoxal Phosphatase| Back To Main Page]] | [[Pyridoxal Phosphatase structure| Back [Structure]]] | [[BIOL3004 2008| Back to BIOL3004 2008]]

Latest revision as of 07:35, 10 June 2008

                     Pyridoxal 5'-phosphate + H2O Reaction Arrow.jpg pyridoxal + phosphate


General Annotation

Fig 17. General annotation of Pyridoxal Phosphatase. Picture courtesy of UniProt


Known Functions

Amino Acid Metabolism

- transamination

- transsulfuration

- selenoamino acid metabolism

- conversion of typtophan to niacin.


Gluconeogenesis

- Catalyse transamination reactions for gluconeogenesis.

- Coenzyme for glycogen phosphorylase, which catalyse glycogenolysis.


Neurotransmitter Synthesis

- Coenzyme for Pyridoxal phosphate-dependent enzymes, which catalyses the biosynthesis of serotonin, epinephrine, norepinephrine and gamma-aminobutyric acid(The four important neurotranmsitters).


Histamine Synthesis

- Metabolism of histamine.


Hemoglobin Synthesis and Function

- Synthesis of heme

- Binds to two sites on hemoglobin, enhancing the oxygen binding of hemoglobin.


Gene Expression

- Increases or decreases the expression of certain genes.

Sequence homology

Fig 18. Results from FASTA showing five highly related enzymes to 2cfsA


Structural Similarities

Fig 19. Search results using Interpro for similar protein structure based on motifs.



































Location of Gene on Genome

Fig 20. Gene location results from Profunc showing two neighbouring genes of 2cfsA with very different sequence but similar function.








































Functional Expression in Tissue

Fig 21. Tissue specific expression of the human PLP phosphatase mRNA. The human multiple tissue expression array was hybridized with human PLP phosphatase-specific DNA probe. Tissue sources for the RNA are indicated below the dot.

Protein-Protein Interactions

String.png
Fig 22. Diagram on protein-protein interaction of 2cfsA.


Functional Prediction:

Cofactor of HSPD1 and play a role in protein folding.

Coenzyme for asparagine synthetase by interacting with TS11.


Back To Main Page | Back [Structure] | Back to BIOL3004 2008

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