2i2O Protein Structure Presentation: Difference between revisions
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'''PDB''' | '''PDB''' | ||
[[Image:1hu3.jpg|thumb|'''Figure 3.0''' Structure of MIF4G (PDB:1hu3)]] | |||
- Protein was isolated form ''Danio rerio'' and expressed in ''Escherichia coli''. | - Protein was isolated form ''Danio rerio'' and expressed in ''Escherichia coli''. |
Revision as of 01:55, 12 June 2007
Materials and Methods
1) Protein search on PDB, NCBI Entrez, Pfam and InterPro databases
- PDB code used: 2i2o
2) Identifying other similar structures with DALI server
3) Comparing the identified structures using CE comparison
Results
PDB
- Protein was isolated form Danio rerio and expressed in Escherichia coli.
- Structure has similarities with the “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein.
- Protein has 2 chains; and consists of nickel (Ni2+) and selenomethionine (C5H11NO2Se) components.
Pfam and InterPro
- Identified as "Middle domain of eIF4G", termed MIF4G.
- Consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.
DALI results
- SUMMARY: PDB/chain identifiers and structural alignment statistics
NR. STRID1 STRID2 Z RMSD LALI LSEQ2 %IDE REVERS PERMUT NFRAG TOPO PROTEIN 1: 3028-A 2i2o-A 37.1 0.0 206 206 100 0 0 1 S STRUCTURAL GENOMICS, UNKNOWN FUNCTION eif4g-like prote 2: 3028-A 1hu3-A 15.2 2.7 164 204 23 0 0 12 S TRANSLATION eif4gii fragment (eukaryotic initiation f 3: 3028-A 1uw4-B 10.9 3.6 164 247 9 0 0 12 S NONSENSE MEDIATED MRNA DECAY PROTEIN regulator of nons 4: 3028-A 1h6k-A 10.6 3.9 175 728 11 0 0 11 S NUCLEAR PROTEIN cbp80 fragment (ncbp 80 kda subunit, 5: 3028-A 2db0-A 8.1 3.3 148 239 14 0 0 13 S PROTEIN BINDING 253aa long hypothetical protein (hypot 6: 3028-A 1b3u-A 8.0 27.7 150 588 13 0 0 13 S SCAFFOLD PROTEIN protein phosphatase pp2a fragment
CE Structural comparison
- 1hu3 and 2i2o: Alpha-helices between 2i2o and 1hu3 were folded in same orientation
- 1h6k and 2i2o: N-terminal region of 1h6k displayed similarity with 2i2o; hence a possibility that MIF4G protein is a region, or even an active domain, near the N-terminal of the nuclear cap binding protein complex (CBC).