DnaB Helicase

protein sequence E.coli K12

dnaB Helicase

H.pylori dnaB helicase

Quench flow method

Different bacteria has different dnaB helicase protein sequence
e.g.
dnaB helicase in E. coli K12
number of aa = 471

       1 magnkpfnkq qaeprerdpq vaglkvpphs ieaeqsvlgg lmldnerwdd vaervvaddf
      61 ytrphrhift emarlqesgs pidlitlaes lerqgqldsv ggfaylaels kntpsaanis
     121 ayadivrera vvremisvan eiaeagfdpq grtsedlldl aesrvfkiae srankdegpk
     181 niadvldatv arieqlfqqp hdgvtgvntg yddlnkktag lqpsdliiva arpsmgkttf
     241 amnlvenaam lqdkpvlifs lempseqimm rslaslsrvd qtkirtgqld dedwarisgt
     301 mgillekrni yiddssgltp tevrsrarri arehggigli midylqlmrv palsdnrtle
     361 iaeisrslka lakelnvpvv alsqlnrsle qradkrpvns dlresgsieq dadlimfiyr
     421 devyhensdl kgiaeiiigk qrngpigtvr ltfngqwsrf dnyagpqydd e

dnaB helicase in H. pylori J99
number of aa = 486

       1 mdhlkhlqql qnierivlsg ivlanhkiee ihsvlepsdf yypphglffe ialklhevnc
      61 pidenfirqk mpkdkqised dlvaifaasp idnieayvee iknasikrkl ftlantireq
     121 alesaqkssd ilnaverevy allngstieg frgikevles tmnlitenqr kgslkvtgip
     181 tgfvqldnyt sgfnqgslvi lgarpsmgkt slmmnmvlsa lnddrgvavf slemsaeqla
     241 lralsdltsi nmhdlesarl dddqwenlak cfdhlsqkkl ffydksyvrm dqirlqlrkl
     301 ksqhkelgia fidylqlmsg nkatkerheq iaeisrelkt lareleipii alvqlnrsle
     361 nrddkrpils dikdsggieq dadivlflyr gyiyqmraed nkidklkkeg kveeaqelhl
     421 kvneerrihk qngsieeaei ivaknrngat gtvytrfnap ftryedmpvd shleegqetk
     481 femptt

dnaB helicase in Geobacillus stearothermophilus
number of aa = 454

       1 mselfserip pqsieaeqav lgavfldpaa lvpaseilip edfyraahqk ifhamlrvad
      61 rgepvdlvtv taelaaseql eeiggvsyls eladavptaa nveyyarive eksvlrrlir
     121 tatsiaqdgy tredeidvll deadrkimev sqrkhsgafk nikdilvqty dniemlhnrd
     181 geitgiptgf teldrmtsgf qrsdliivaa rpsvgktafa lniaqnvatk tnenvaifsl
     241 emsaqqlvmr mlcaegnina qnlrtgkltp edwgkltmam gslsnagiyi ddtpsirvsd
     301 irakcrrlkq esglgmivid ylqliqgsgr skenrqqevs eisrslkala relevpvial
     361 sqlsrsveqr qdkrpmmsdi resgsieqda divaflyrdd yynkdsenkn iieiiiakqr
     421 ngpvgtvqla fikeynkfvn lerrfdeaqi ppga

22 different aa and its abbrev.
Alanine--------------A---------Ala
Cysteine------------C---------Cys
Aspartic acid------D---------Asp
Glutamic acid-----E---------Glu
Phenylalanine-----F---------Phe
Glycine--------------G---------Gly
Histidine------------H---------His
Isoleucine-----------I---------Ile
Lysine---------------K---------Lys
Leucine-------------L---------Leu
Methionine--------M---------Met
Asparagine--------N---------Asn
Pyrrolysine--------O---------Pyl
Proline--------------P---------Pro
Glutamine---------Q---------Gln
Arginine------------R---------Arg
Serine---------------S---------Ser
Threonine----------T---------Thr
Selenocysteine---U---------Sec
Valine---------------V---------Val
Tryptophan--------W--------Trp
Tyrosine------------Y---------Tyr

--ThomasHuber 09:37, 19 February 2008 (EST) [[Image:[[Image:

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