2i2O Protein Structure Presentation
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Materials and Methods
1) Protein search on PDB, NCBI Entrez, Pfam and InterPro databases
- PDB code used: 2i2o
2) Identifying other similar structures with DALI server
3) Comparing the identified structures using CE comparison
Results
PDB
- Protein was isolated form Danio rerio and expressed in Escherichia coli.
- Structure has similarities with the “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein.
- Protein has 2 chains; and consists of nickel (Ni2+) and selenomethionine (C5H11NO2Se) components.
Pfam and InterPro
- Identified as "Middle domain of eIF4G", termed MIF4G.
- Consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.
DALI results