2i2O Protein Structure Presentation: Difference between revisions
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'''Materials and Methods''' | '''Materials and Methods''' | ||
1)Protein search on PDB, NCBI Entrez, Pfam and InterPro databases | 1) Protein search on PDB, NCBI Entrez, Pfam and InterPro databases | ||
* PDB code used: 2i2o | * PDB code used: 2i2o | ||
2)Identifying other similar structures with DALI server | 2) Identifying other similar structures with DALI server | ||
3)Comparing the identified structures using CE comparison | 3) Comparing the identified structures using CE comparison | ||
'''Results''' | |||
'''PDB''' | |||
- Protein was isolated form ''Danio rerio'' and expressed in ''Escherichia coli''. | |||
- Structure has similarities with the “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein. | |||
- Protein has 2 chains; and consists of nickel (Ni2+) and selenomethionine (C5H11NO2Se) components. | |||
'''Pfam and InterPro''' | |||
- Identified as "Middle domain of eIF4G", termed MIF4G. | |||
- Consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA. | |||
'''DALI results''' | |||
Revision as of 01:47, 12 June 2007
Materials and Methods
1) Protein search on PDB, NCBI Entrez, Pfam and InterPro databases
- PDB code used: 2i2o
2) Identifying other similar structures with DALI server
3) Comparing the identified structures using CE comparison
Results
PDB
- Protein was isolated form Danio rerio and expressed in Escherichia coli.
- Structure has similarities with the “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein.
- Protein has 2 chains; and consists of nickel (Ni2+) and selenomethionine (C5H11NO2Se) components.
Pfam and InterPro
- Identified as "Middle domain of eIF4G", termed MIF4G.
- Consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.
DALI results
