2i2O Protein Structure Presentation

Materials and Methods

1) Protein search on PDB, NCBI Entrez, Pfam and InterPro databases

• PDB code used: 2i2o

2) Identifying other similar structures with DALI server

3) Comparing the identified structures using CE comparison

Results

PDB

- Protein was isolated form Danio rerio and expressed in Escherichia coli.

- Structure has similarities with the “Eukaryotic Translation Initiation Factor 4G (eIF4G)” protein.

- Protein has 2 chains; and consists of nickel (Ni2+) and selenomethionine (C5H11NO2Se) components.

Pfam and InterPro

- Identified as "Middle domain of eIF4G", termed MIF4G.

- Consists essentially of alpha helices and has “multiple alpha-helical repeats” (reference). Within eIF4G, it binds to the RNA helicase eIF4A, eIF3, RNA and DNA.

DALI results